Eptesicus fuscus Kidney Cell Line (Efk3B)
Immortalized Eptesicus fuscus (Big Brown Bat) cell line (Efk3B) can be used to successfully cultivate viruses from the families Coronaviridae, Filoviridae, Rhabdoviridae, Paramyxoviridae and Herpesviridae.
Highlights:
- Immortalized using a novel technology, Myotis polyomavirus large T-antigen
- Capable to grow MERS-Coronavirus, PED-Coronavirus, Rabies, Ebola virus, Vesicular Stomatitis Virus, Herpes Simplex Virus and Henipaviruses
The big brown bat (Eptesicus fuscus) is native to North America, Central America, the Caribbean, and extreme northern South America.
From the laboratory of Vikram Misra, PhD, University of Saskatchewan.
Immortalized Eptesicus fuscus (Big Brown Bat) cell line (Efk3B) can be used to successfully cultivate viruses from the families Coronaviridae, Filoviridae, Rhabdoviridae, Paramyxoviridae and Herpesviridae.
Highlights:
- Immortalized using a novel technology, Myotis polyomavirus large T-antigen
- Capable to grow MERS-Coronavirus, PED-Coronavirus, Rabies, Ebola virus, Vesicular Stomatitis Virus, Herpes Simplex Virus and Henipaviruses
The big brown bat (Eptesicus fuscus) is native to North America, Central America, the Caribbean, and extreme northern South America.
From the laboratory of Vikram Misra, PhD, University of Saskatchewan.
Specifications
| Product Type: | Cell Line |
| Name: | Efk3B |
| Cell Type: | Epithelial |
| Accession ID: | CVCL_GZ34 |
| Morphology: | Variable - round, cobweb-like at first then becoming epithelial-like |
| Source: | Kidney |
| Organism: | Eptesicus fuscus (Big Brown Bat) |
| Biosafety Level: | BSL1 |
| Growth Conditions: | 37 °C, 5% CO2 Grown in D-MEM with GlutaGRO (Corning), 10% FBS, 1% GlutaMAX (Gibco), Pen/Strep. NOTE: for passaging, use 0.25% trypsin |
| Cryopreservation: | Complete medium with 10% DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
Provider
From the laboratory of Vikram Misra, PhD, University of Saskatchewan.
References
- Subudhi, S., Rapin, N., Dorville, N., Hill, J.E., Town, J., Willis, C.K.R., Bollinger, T.K., Misra, V., 2018. Isolation, characterization and prevalence of a novel Gammaherpesvirus in Eptesicus fuscus, the North American big brown bat. Virology 516, 227-238.
- Banerjee A, Rapin N, Bollinger T, Misra V. Lack of inflammatory gene expression in bats: a unique role for a transcription repressor. Sci Rep. 2017 May 22;7(1):2232. doi: 10.1038/s41598-017-01513-w. PubMed PMID: 28533548; PubMed Central PMCID: PMC5440382.
- Banerjee A, Rapin N, Miller M, Griebel P, Zhou Y, Munster V, Misra V. Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen. J Virol Methods. 2016 Sep 14. doi: 10.1016/j.jviromet.2016.09.008.
- Gonçalves-Carneiro D, Takata MA, Ong H, Shilton A, Bieniasz PD. Origin and evolution of the zinc finger antiviral protein. PLoS Pathog. 2021 Apr 26;17(4):e1009545. View article
If you publish research with this product, please let us know so we can cite your paper.
