Anti-CD60 [UM4D4] Antibody
This mouse IgM monoclonal antibody was generated against human rheumatoid synovial T cell line (ST-1). It has been classified as recognizing the CD60 structure on a T cell subset and on various other cells, such as stromal cells in thymus and synovium. The antibody recognizes a carbohydrate-dependent epitope on proteins of 92 and 70 kDa, and also binds to the 9-O-acetylated GD3 ganglioside.
CD60b (formally called CDw60) is a 9-O-acetylated form of GD3 ganglioside. The CD60b antigen is expressed on peripheral blood T cells, monocytes, platelets, synovial fibroblasts and some cell lines. Cytotoxic T cells, T helper cells and IL-4-producing cells are the T cell subsets that express CD60b. It is hypothesized that CD60b may provide costimulatory signals for T cells. After stimulation of naive CD45RA+ T cells, the CD60b antigen is upregulated, corresponding with the switch from CD45RA+ to CD45RO+. CD34+ hemato-poietic precursor cells have been shown to be CD60b positive.
From the laboratory of David A. Fox, MD, University of Michigan.
Part of The Investigator's Annexe program.
This mouse IgM monoclonal antibody was generated against human rheumatoid synovial T cell line (ST-1). It has been classified as recognizing the CD60 structure on a T cell subset and on various other cells, such as stromal cells in thymus and synovium. The antibody recognizes a carbohydrate-dependent epitope on proteins of 92 and 70 kDa, and also binds to the 9-O-acetylated GD3 ganglioside.
CD60b (formally called CDw60) is a 9-O-acetylated form of GD3 ganglioside. The CD60b antigen is expressed on peripheral blood T cells, monocytes, platelets, synovial fibroblasts and some cell lines. Cytotoxic T cells, T helper cells and IL-4-producing cells are the T cell subsets that express CD60b. It is hypothesized that CD60b may provide costimulatory signals for T cells. After stimulation of naive CD45RA+ T cells, the CD60b antigen is upregulated, corresponding with the switch from CD45RA+ to CD45RO+. CD34+ hemato-poietic precursor cells have been shown to be CD60b positive.
From the laboratory of David A. Fox, MD, University of Michigan.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | CD60 |
| Molecular Weight: | 92/70 kDa |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | UM4D4 |
| Reactivity: | Human |
| Immunogen: | RA synovial T cells |
| Species Immunized: | Mouse |
| Epitope: | Carbohydrate-dependent epitope |
| Buffer: | Unpurified supernatant |
| Tested Applications: | Flow Cytometry, IP, WB |
| Storage: | -80C |
| Shipped: | Cold Packs |
- Higgs JB, Zeldes W, Kozarsky K, Schteingart M, Kan L, Bohlke P, Krieger K, Davis W, Fox DA. A novel pathway of human T lymphocyte activation. Identification by a monoclonal antibody generated against a rheumatoid synovial T cell line. J Immunol. 1988 Jun 1;140(11):3758-65.
- Fox DA, Millard JA, Kan L, Zeldes WS, Davis W, Higgs J, Emmrich F, Kinne RW. Activation pathways of synovial T lymphocytes. Expression and function of the UM4D4/CDw60 antigen. J Clin Invest. 1990 Oct;86(4):1124-36.
- Fox DA, He X, Abe A, Hollander T, Li LL, Kan L, Friedman AW, Shimizu Y, Shayman JA, Kozarsky K. The T lymphocyte structure CD60 contains a sialylated carbohydrate epitope that is expressed on both gangliosides and glycoproteins. Immunol Invest. 2001 May;30(2):67-85.
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