Anti-Bat Ig Light Chain [BT1-4F10] Hybridoma

This hybridoma line constitutively produces a mouse anti-bat Ig Light chain monoclonal antibody.

Highlights:

  • Binds to B cells and serum immunoglobulin by virtue of specificity for Ig Light Chain
  • Reactivity demonstrated for E. fuscus and additional microchiroptera
  • No reactivity against Ig from megachiroptera has been demonstrated to date

The hybridoma was generated by fusion of splenocytes from a BALB/c mouse that had been immunized with big brown bat (Eptisicus fuscus) splenocytes. The resulting hybridomas were plated in methylcellulose and subsequently subcloned in liquid culture.

From the laboratory of William T. Lee, PhD, Health Research Incorporated

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EHR001
Anti-Bat Ig Light Chain [BT1-4F10] Hybridoma
1 vial In stock
Regular Price:$1,120.00
On Sale:
Specifications

Product Type: Antibody
Cell Type: Hybridoma cell line
Antigen: Immunoglobulin Light Chain
Isotype: IgG1
Clonality: Monoclonal
Clone Name: BT1-F10
Reactivity: Microchiropteran Bats, no reactivity against megachiroptera
Growth Conditions: Complete RPMI (RPMI-1640 with 10% fetal bovine serum and supplemented with 50 uM 2-mercaptoethanol, 100U/ml penicillin, 100 ug/ml streptomycin, and 2mM glutamine)
Immunogen: E. fuscussplenocytes
Species Immunized: BALB/c mouse
Cryopreservation: Resuspend cells directly into 90% FBS, 10% DMSO. Store vials at -80C for 24-48 hrs at -70 before transfer to liquid nitrogen.
Storage: Liquid nitrogen
Shipped: Dry ice

Documentation

Mouse-Anti-Bat Ig Light Chain Hybridoma

Provider
From the laboratory of William T. Lee, PhD, Health Research Incorporated
References

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