Anti-Profilin-1 [mAbPRF12a] Antibody
![Anti-Actin-2 [mAb13a] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/778_200120200126217770D.jpg "Anti-Profilin-1 [mAbPRF12a] Antibody")
This mouse monoclonal antibody was generated against plant Profilin (PRF1) and reacts strongly with arabidopsis PRF1 and PRF2 and weakly with PRF3.
Profilin is a low-molecular weight, actin monomer-binding protein that regulates the organization of actin cytoskeleton in eukaryotes. Profilin promotes actinassembly at the barbed end of filaments and causes depolymerization of filaments by binding and sequestering G-actin. Profilin also interacts with several other ligands, such as membrane polyphospho-inositides, short proline rich stretches in proteins, and the Arp 2/3 complex.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Part of The Investigator's Annexe program.
This mouse monoclonal antibody was generated against plant Profilin (PRF1) and reacts strongly with arabidopsis PRF1 and PRF2 and weakly with PRF3.
Profilin is a low-molecular weight, actin monomer-binding protein that regulates the organization of actin cytoskeleton in eukaryotes. Profilin promotes actinassembly at the barbed end of filaments and causes depolymerization of filaments by binding and sequestering G-actin. Profilin also interacts with several other ligands, such as membrane polyphospho-inositides, short proline rich stretches in proteins, and the Arp 2/3 complex.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | Profilin, PRF1 |
| Accession ID: | P07737, AEC06923 |
| Molecular Weight: | 14 kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | mAbPRF12a (2-11-H7) |
| Reactivity: | Arabidopsis Profilin |
| Immunogen: | Purified plant Profilin 1 (PRF1) |
| Species Immunized: | Mouse |
| Purification Method: | Protein G column |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB (1-2ug/mL), IF |
| Storage: | -20C |
| Shipped: | Cold packs |
| Antibody | Reactivity |
| Profilin-1 [mAbPRF1a] | PRF1 |
| Profilin-1 [mAbPRF12a] | PRF1, 2 (weak binding to PRF3) |
| Profilin-4 [mAbPRF45a] | PRF4, 5 |
Western Blot Analysis
Blot contained 5g of crude arabidopsis recombinant profilins and 20g of total protein from whole plant extract. Coomassie blue stained gel (bottom) shows equal loading of recombinant profilins.
Adapted from: Kandasamy, M.K., et al. (2002) Cell Motil Cytoskeleton 52, 22-32.
- Kandasamy, M.K., McKinney, E.C., and Meagher, R.B. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52, 22-32.
- McKinney, E.C., Kandasamy, M.K., and Meagher, R.B. (2001). Small changes in the regulation of one arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13, 1179-91.
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