S-nitrosothiol reactive zwitterionic and pI balancing Cy5 dye (Z-Cy5 dye). This reagent can be used to attach Cy5 fluorophore to proteins and peptides containing S-nitrosothiol modified cysteine residues.
Highlights:
From the laboratories of Edward A. Dratz, PhD, and Paul A. Grieco, PhD, Montana State University.
Part of The Investigator's Annexe program.
Product Type: | Small Molecule |
Name: | Z-Cy5 Thiol |
Chemical Formula: | C42H57N5O6S2 |
Molecular Weight: | 792.1 |
Format: | Dark blue powder |
Purity: | 95+% (NMR, HPLC) |
Solubility: | Good in water and polar organic solvents |
Spectral Information: | Excitation maximum: 642nmExtinction at max excitation: 150,000Emission maximum: 662nmFluorescence quantum yield: 0.45 |
Storage: | -20C. Protect from light. Dessicate. |
Shipped: | Cold packs |
Suggested amount: 2nmol/50ug protein.
2D DIGE Examples
2D gel of total soluble protein from Sulfolobus solfataricus labeled with Z-CyDyes. Each dye was used to label the protein sample separately. Dye labeled protein samples were combined and run on the same gel. After separation, the gel was scanned with 100 ?m steps using the following laser excitation/band-pass filter combinations: 488/520 nm for Z-Cy2 (left), 532/580 nm for Z-Cy3 (middle), and 633/670 nm for Z-Cy5 (right). Images show the relatively crowded central region of the gel (pI 4?9 and 15?150 kDa), revealing well-defined spots with all three dyes.
Adapted from: Epstein MG, et al. Bioconjug Chem. 2013 Sep 18;24(9):1552-61.
Comparison of Total Spot Number
Total number of spots detected from a gel using Z-Cy2, ZCy3, and Z-Cy5 and from a matched gel using CyDye DIGE Fluor minimal Cy2, Cy3, and Cy5 purchased from GE Healthcare. The protein samples labeled and experimental protocols were the same for both gels and all dyes. Progenesis SameSpots software detects more spots when Z-CyDyes are used in comparison to CyDyes.
Adapted from: Epstein MG, et al. Bioconjug Chem. 2013 Sep 18;24(9):1552-61.
Looking for a different Z-Cy Dye? Check out our other Z-Cy Dyes!
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