SACK-Xs Lig-13 Clonal Adult Rat Cholangiocytic Stem Cells
Growing and expanding primary, differentiated adult cells in culture is difficult and laborious because many differentiated cells can only be maintained in vitro for a limited period of time. However, for controlled experiments, it may be necessary to maintain a consistent population of cells.
Maintaining a population of adult stem cells (ASCs), allows one to exponentially propagate a population of cells in vitro, and induce differentiation when desired. This is possible with SACK cell strains and specified cell culture conditions.
SACK ASC cell properties – Long-term propagation in xanthosine (Xs)-supplemented medium
- Suppressed asymmetric cell kinetics
Differentiated progeny cell properties – Xs-free medium supplemented with 10% rat serum, confluent monolayer; or serum reduction and Xs-free medium supplemented with 10% bovine serum
Biliary Properties
- Albumin expression, but no secretion
- Alpha-fetoprotein secretion
- CK7 expression (biliary epithelial cell marker)
- Form tubules with polarized cells (e.g., luminal microvilli)
*Additional similar, but less well characterized, independent clonal cell strains are available. For more information, contact us.
Growing and expanding primary, differentiated adult cells in culture is difficult and laborious because many differentiated cells can only be maintained in vitro for a limited period of time. However, for controlled experiments, it may be necessary to maintain a consistent population of cells.
Maintaining a population of adult stem cells (ASCs), allows one to exponentially propagate a population of cells in vitro, and induce differentiation when desired. This is possible with SACK cell strains and specified cell culture conditions.
SACK ASC cell properties – Long-term propagation in xanthosine (Xs)-supplemented medium
- Suppressed asymmetric cell kinetics
Differentiated progeny cell properties – Xs-free medium supplemented with 10% rat serum, confluent monolayer; or serum reduction and Xs-free medium supplemented with 10% bovine serum
Biliary Properties
- Albumin expression, but no secretion
- Alpha-fetoprotein secretion
- CK7 expression (biliary epithelial cell marker)
- Form tubules with polarized cells (e.g., luminal microvilli)
*Additional similar, but less well characterized, independent clonal cell strains are available. For more information, contact us.
| Product Type: | Cell Line |
| Name: | SACK-Xs Lig-13 |
| Cell Type: | adult rat cholangiocytic stem cells |
| Accession ID: | CVCL_5K98 |
| Morphology: | epitheliod |
| Source: | liver; derived from adult male Fischer 344 rats |
| Organism: | rat |
| Biosafety Level: | Bsl-1 |
| Growth Conditions: | SACK medium - DMEM (4.5mg/ml high glucose) w/ 10% dialyzed FBS and 400?M Xanthosine |
| Subculturing: | Passage 1:5 when cells reach approx. 80% confluence |
| Cryopreservation: | 70% DMEM (high glucose); 20% dialyzed FBS; 10% DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
Replacement of the SACK medium with differentiation conditions allows the expanded tissue stem cells to regain their in vivo homeostatic state of asymmetric self-renewal, which yields cells committed to tissue-specific differentiation.
- Sherley et al. (2010) Hepatocyte precursor cell lines. Patent No. US 7,645,610 B2.
- Paré, J.-F. and Sherley, J. L. (2006) Biological principles for ex vivoadult stem cell expansion, in Curr. Top. in Devel. Biol., ed. G.Schatten, Elsevier, Inc. (San Diego), Vol. 73, pp. 141-171.
- Lee et al. (2003) Clonal expansion of adult rat liver epithelial stemcells by suppression of asymmetric cell kinetics (SACK). Biotech. &Bioeng. 83, 760-771.
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