Anti-Prothrombin [29J3-62] Antibody
![Anti-Prothrombin [29J3-62] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/826_200120200126238250.jpg "Anti-Prothrombin [29J3-62] Antibody")
This mouse monoclonal antibody (clone 29J3-62) is specific for human prothrombin. 29J3-62 has a high affinity for both soluble prothrombin and prothrombin bound to phospholipid or apoptotic cells, and represents a prototype for a phospholipid-independent antiphospholipid antibody.
Highlights:
- Shows high affinity for soluble prothrombin
- Also reacts with prothrombin bound to phospholipid or apoptotic cells
- Shown to enhance binding of prothrombin to apoptotic cells
- Acts as an antiphospholipid antibody that can bind to prothrombin in the absence or presence of phospholipid
- Possesses lupus anticoagulant activity - useful as positive control in in vitro coagulation assays
?Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin and factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of prothrombin (factor II) leads to formation of thrombin, which activates factors V, VII, VIII and XIII in complex with thrombomodulin and protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.
From the laboratory of Joyce Rauch, PhD, McGill University.
Part of The Investigator's Annexe program.
This mouse monoclonal antibody (clone 29J3-62) is specific for human prothrombin. 29J3-62 has a high affinity for both soluble prothrombin and prothrombin bound to phospholipid or apoptotic cells, and represents a prototype for a phospholipid-independent antiphospholipid antibody.
Highlights:
- Shows high affinity for soluble prothrombin
- Also reacts with prothrombin bound to phospholipid or apoptotic cells
- Shown to enhance binding of prothrombin to apoptotic cells
- Acts as an antiphospholipid antibody that can bind to prothrombin in the absence or presence of phospholipid
- Possesses lupus anticoagulant activity - useful as positive control in in vitro coagulation assays
?Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin and factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of prothrombin (factor II) leads to formation of thrombin, which activates factors V, VII, VIII and XIII in complex with thrombomodulin and protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.
From the laboratory of Joyce Rauch, PhD, McGill University.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | Prothrombin (Coagulation factor II) |
| Accession ID: | P00734 |
| Isotype: | 70.037 kDa (theoretical);IgG1k |
| Clonality: | Monoclonal |
| Clone Name: | 29J3-62 |
| Reactivity: | Human |
| Immunogen: | Human prothrombin |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, pH 7.4 |
| Tested Applications: | ELISA (1:5000 or 0.2 ug/mL) |
| Concentration: | 1 mg/mL |
| Storage: | -20C |
| Shipped: | Cold packs |
- D'Agnillo P, Levine JS, Subang R, Rauch J. Prothrombin binds to the surface of apoptotic, but not viable, cells and serves as a target of lupus anticoagulant autoantibodies. J Immunol. 2003 Mar 15;170(6):3408-22.
- Rauch J, D'Agnillo P, Subang R, Levine JS. Anti-phospholipid antibodies (aPL) and apoptosis: prothrombin-dependent aPL as a paradigm for phospholipid-dependent interactions with apoptotic cells. Thromb Res. 2004; 114(5-6): 371-382.
- Roggenbuck D, Borghi MO, Somma V, Büttner T, Schierack P, Hanack K, Grossi C, Bodio C, Macor P, von Landenberg P, Boccellato F, Mahler M, Meroni PL. Antiphospholipid antibodies detected by line immunoassay differentiate amongpatients with antiphospholipid syndrome, with infections and asymptomatic carriers. Arthritis Res Ther. 2016 May 21;18(1):111. View Article
If you publish research with this product, please let us know so we can cite your paper.
