This biotinylated bacterial protein can be used as a procedural control for affinity capture experiments using avidin or streptavidin beads and biotinylated proteins.
Highlights:
Avidin and streptavidin are widely used in biotechnology and molecular research for detection, purification, crosslinking, and labeling of biotinylated targets. This protein can be used in a manner similar to the way prebiotinylated AhpC was used in experiments with DCP-Bio1 (discussed in Klomsiri et al., Methods Enzymol, 2010).
From the laboratory of Leslie B. Poole, PhD, Wake Forest School of Medicine.
Part of The Investigator's Annexe program.
Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Product Type: | Protein |
Name: | Biotinylated-Trx Loading Control Protein |
Accession ID: | P10599, P0AA25 |
Source: | Recombinantly expressed in E.Coli and modified with biotin-maleimide |
Molecular Weight: | 11,675 + 903 Da (2 biotin molecules) |
Amino Acid Sequence: | SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA |
Fusion Tag(s): | None |
Purity: | 95% as determined by SDS-PAGE |
Buffer: | 25mM potassium phosphate pH 7, 1mM EDTA |
Concentration: | 1mg/mL |
Suggested Amount per Experiment: | 1 microgram per 1000 micrograms of sample |
Comments: | Has 2 biotin-maleimide moieties attached at Cys residues |
Storage: | -20C (avoid repeated freeze-thaw cycles) |
Shipped: | Cold packs |
If you publish research with this product, please let us know so we can cite your paper.