TAMRA-Azide-Biotin
A trifunctional biotinylation reagent that can be used for both visualization and affinity purification of alkyne-tagged molecules.
Highlights:
- Trifunctional – allows for both visualization and affinity purification of alkyne-tagged proteins
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
TAMRA-Azide-Biotin incorporates an azido group to enable click chemistry-mediated labeling of alkyne-tagged proteins. A hydophilic PEG spacer increases solubility in aqueous media and reduces steric hindrance when binding to streptavidin molecules. The biotin enables efficient protein enrichment. Finally, a carboxytetramethylrhodamine (TAMRA) fluorophore, which remains attached to the protein after elution from streptavidin resin, serves the purpose of sensitive detection of tagged proteins.
A trifunctional biotinylation reagent that can be used for both visualization and affinity purification of alkyne-tagged molecules.
Highlights:
- Trifunctional – allows for both visualization and affinity purification of alkyne-tagged proteins
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
TAMRA-Azide-Biotin incorporates an azido group to enable click chemistry-mediated labeling of alkyne-tagged proteins. A hydophilic PEG spacer increases solubility in aqueous media and reduces steric hindrance when binding to streptavidin molecules. The biotin enables efficient protein enrichment. Finally, a carboxytetramethylrhodamine (TAMRA) fluorophore, which remains attached to the protein after elution from streptavidin resin, serves the purpose of sensitive detection of tagged proteins.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Name: | TAMRA-Azide-Biotin |
| Chemical Formula: | C57H79N11O14S |
| Molecular Weight: | 1174.37 |
| Extinction Coefficient: | 92000 M-1cm-1 |
| Variant MPN: | 1048 |
| Format: | Red amorphous solid |
| Solubility: | DMSO, DMF, water, MeOH |
| Spectral Information: | Abs/Em = 546/565 nm |
| Storage: | -20C |
| Shipped: | Ambient |
Documentation
References
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