Desthiobiotin-PEG3-Azide
An azide-activated desthiobiotin labeling reagent with a hydrophilic spacer arm.
Highlights:
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Desthiobiotin – allows easy elution from streptavidin, an ideal feature for affinity purification applications
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
- Extended PEG3 spacer – the PEG spacer arm is hydrophilic and provides labeled molecules with greater solubility to compared to reagents having only hydrocarbon spacers
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
An azide-activated desthiobiotin labeling reagent with a hydrophilic spacer arm.
Highlights:
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Desthiobiotin – allows easy elution from streptavidin, an ideal feature for affinity purification applications
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
- Extended PEG3 spacer – the PEG spacer arm is hydrophilic and provides labeled molecules with greater solubility to compared to reagents having only hydrocarbon spacers
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Small Molecule |
| Name: | Desthiobiotin-PEG3-Azide |
| Chemical Formula: | C18H34N6O5 |
| Molecular Weight: | 414.5 |
| Variant MPN: | 1107 |
| Format: | Colorless to slightly yellow oil |
| Solubility: | DMSO, DMF, MeOH |
| Storage: | -20C. Desiccate |
| Shipped: | Ambient |
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