E. coli Strain ALS1058
Escherichia coli cells cannot metabolize glucose.
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
Part of The Investigator's Annexe program.
Escherichia coli cells cannot metabolize glucose.
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
Part of The Investigator's Annexe program.
Specifications
| Product Type: | Bacteria |
| Name: | ALS1058 |
| Cell Type: | Bacteria |
| Strain: | ALS1058 |
| Organism: | Escherichia coli |
| Genotype: | Hfr zbi::Tn10 poxB1 Δ(aceEF) rpsLpps-4 pfl-1 ldhA::Kan atpFH::Cam |
| Antibiotic Resistance: | Kanamycin, chloramphenicol |
| Growth Conditions: | TYA medium, 10g/L tryptone, 5g/L NaCl, 1g/L yeast extract, 1.36 g/L sodium acetate trihydrate Na(CH3COO)?3H2O, pH adjusted to 7.0, aerobic 37C |
| Format: | Lyophilized culture |
| Storage: | Room temperature as lyophilized culture |
| Shipped: | Ambient temperature |
Documentation
Protocol Notes
- Inject 1 mL of sterile DI water into vial and gently mix.
- Use small portions of vial (liquid) contents to inoculate a sterile, liquid culture to an initial OD of 0.03-0.1. For this liquid culture, use the specified growth medium with or without an antibiotic as appropriate.
- When culture has visibly grown in liquid medium, plate on solid (Agar) growth medium and incubate at 37C. Maintain strain.
Research
| Strain | Phenotype |
| ALS974 | Accumulates lactic acid |
| ALS929 | Accumulates pyruvic acid |
| ALS1392 | Does not metabolize arabinose, glucose nor xylose |
| ALS1391 | Does not metabolize arabinose nor xylose |
| ALS1371 | Does not metabolize arabinose nor glucose |
| ALS1370 | Does not metabolize xylose nor glucose |
| ALS1074 | Does not metabolize xylose but can accumulate lactic acid from glucose |
| ALS1073 | Does metabolize glucose but can accumulate lactic acid from xylose |
| ALS1060 | Does not metabolize xylose nor glucose |
| ALS1059 | Accumulates pyruvic acid |
| ALS1058 | Does not metabolize glucose |
| ALS1048 | Does not metabolize glucose |
| ALS1038 | Does not metabolize xylose |
| ALS1054 | Accumulates pyruvic acid |
| ALS1122 | Does not metabolize xylose nor glucose |
Provider
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
References
- Zhu Y, Eiteman MA, Altman R, Altman E. High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain. Appl Environ Microbiol. 2008 Nov;74(21):6649-55.
- US Patent Number 8,551,758; PCT Publication number WO2008124162 A2. Pending applications in several jurisdictions University of Georgia Research Foundation, Inc.
If you publish research with this product, please let us know so we can cite your paper.
