UV Cleavable Biotin-Azide
The UV Cleavable Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.
Highlights:
- Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
- Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
- Also available: 13C6-UV-Biotin-azide
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
The UV Cleavable Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.
Highlights:
- Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
- Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
- Also available: 13C6-UV-Biotin-azide
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
Specifications
| Product Type: | Small Molecule |
| Name: | UV Cleavable Biotin-Azide |
| Chemical Formula: | C38H51N7O9S |
| Source: | Synthetic |
| Molecular Weight: | 781.92 |
| Format: | Solid |
| Solubility: | DMSO, MeOH |
| Photolysis: | λ=365nm |
| Storage: | -20C |
| Shipped: | Ambient temperature |
Data
Cellular Click Chemistry Diagram
Provider
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
References
- Yang J, Tallman KA, Porter NA, Liebler DC. Quantitative chemoproteomics for site-specific analysis of protein alkylation by 4-hydroxy-2-nonenal in cells. Anal Chem. 2015 Mar 3;87(5):2535-41. doi: 10.1021/ac504685y. Epub 2015 Feb 9. PMID: 25654326; PMCID: PMC4350606.
- Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems. Codreanu, S. G.; Ullery, J. C.; Zhu, J.; Tallman, K. A.; Beavers, W. N.; Porter, N. A.; Marnett, L. J.; Zhang, B.; Liebler, D. C. Mol. Cell. Proteomics 2014, 13, 849.
- An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-Derived Electrophiles by Streptavidin Catch and Photo-Release. Kim, H.-Y. H.; Tallman, K. A.; Liebler, D. C.; Porter, N. A. Mol. Cell. Proteomics 2009, 8, 2080.
- Yang J, Gupta V, Tallman KA, Porter NA, Carroll KS, Liebler DC. Global, in situ, site-specific analysis of protein S-sulfenylation. Nat Protoc. 2015 Jul;10(7):1022-37. View Article
- Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. View Article
If you publish research with this product, please let us know so we can cite your paper.
