Anti-Dynein Light Chain Tctex-Type 3 (DYNLT3) [10A3] Antibody
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This IgG2a mouse monoclonal antibody was generated against full-length recombinant human dynein light chain Tctex-type 3 (DYNLT3) protein and is reactive against human, mouse and rat DYNLT3.
Highlights
- Reacts with human, mouse and rat dynein light chain Tctex-type 3
- Suitable for Western Blot and Immmunofluorescence
Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins). In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport. Three families of dynein light chains (DYNL) have been identified. Two isoforms of the DYNLT family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes.
From the laboratory of K. Kevin Pfister, PhD, University of Virginia
Part of The Investigator's Annexe program.
This IgG2a mouse monoclonal antibody was generated against full-length recombinant human dynein light chain Tctex-type 3 (DYNLT3) protein and is reactive against human, mouse and rat DYNLT3.
Highlights
- Reacts with human, mouse and rat dynein light chain Tctex-type 3
- Suitable for Western Blot and Immmunofluorescence
Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins). In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport. Three families of dynein light chains (DYNL) have been identified. Two isoforms of the DYNLT family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes.
From the laboratory of K. Kevin Pfister, PhD, University of Virginia
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Alternative Name(s): | RCG42244, isoform CRA_a,Protein Dynlt3 |
| Antigen: | Dynein light chain Tctex-type 3 |
| Accession ID: | P51808 |
| Molecular Weight: | 13 kDa |
| Isotype: | IgG2a |
| Clonality: | Monoclonal |
| Clone Name: | 10A3-CA-81 |
| Reactivity: | Human, mouse and rat |
| Immunogen: | Full-length recombinant human DYNLT3 protein |
| Species Immunized: | Mouse |
| Purification Method: | Protein G Affinity Chromatography |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB, IHC |
| Storage: | -20C |
| Shipped: | Cold packs |
- Ha J, Lo KW, Myers KR, Carr TM, Humsi MK, Rasoul BA, Segal RA, Pfister KK. A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes. J Cell Biol. 2008 Jun 16;181(6):1027-39.
- Lo KW, Kogoy JM, Rasoul BA, King SM, Pfister KK. Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex. J Biol Chem. 2007 Dec 21;282(51):36871-8.
- Lo KW, Kogoy JM, Pfister KK. The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3. J Biol Chem. 2007 Apr 13;282(15):11205-12.
- Myers KR1, Lo KW, Lye RJ, Kogoy JM, Soura V, Hafezparast M, Pfister KK. Intermediate chain subunit as a probe for cytoplasmic dynein function: biochemical analyses and live cell imaging in PC12 cells. J Neurosci Res. 2007 Sep;85(12):2640-7.
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