Anti-Xylan [CCRC-M114] Antibody (supernatant)
![Anti-Xylan [CCRC-M114] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1027_2001202001263110260.jpg "Anti-Xylan [CCRC-M114] Antibody (supernatant)")
This mouse IgM monoclonal (CCRC-M114) was generated against Xylan of Phormium cookianum and recognizes the glycan group of Xylan-3.
Highlights:
- Reacts with wheat, sorghum, corn and Phormium sp. glycan group of Xylan-3
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Xylan is a group of hemicelluloses that reside in plant cells walls and also can be found in some algae (both green and red). Xylans are polysaccharides whose backbone consists of beta-1,4-linked xylosyl residues. This backbone can be substituted with side-chains of arabinosyl, glucuronosyl, and 4-O-mthylglucuronosyl residues, and can also be further modified by acetyl substitution on the hydroxyls of the xylosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia
Part of The Investigator's Annexe program.
This mouse IgM monoclonal (CCRC-M114) was generated against Xylan of Phormium cookianum and recognizes the glycan group of Xylan-3.
Highlights:
- Reacts with wheat, sorghum, corn and Phormium sp. glycan group of Xylan-3
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Xylan is a group of hemicelluloses that reside in plant cells walls and also can be found in some algae (both green and red). Xylans are polysaccharides whose backbone consists of beta-1,4-linked xylosyl residues. This backbone can be substituted with side-chains of arabinosyl, glucuronosyl, and 4-O-mthylglucuronosyl residues, and can also be further modified by acetyl substitution on the hydroxyls of the xylosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Accession ID: | CCRC M114 |
| Antigen: | Xylan of Phormium cookianum |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | CCRC M114 |
| Reactivity: | Wheat, sorghum, corn, Phormium sp. |
| Immunogen: | Xylan of Phormium cookianum |
| Species Immunized: | Mouse |
| Epitope: | alpha-Fuc-(1,2)-beta-Gal |
| Buffer: | Cell culture supernatant, Use undiluted or at 1:10 dilution |
| Tested Applications: | ELISA, Immunolabeling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
CCRC-M114 Cross Reactivity Map
CCRC-M114 binds to Phormium xylans and some monocot xylans, but not to dicot xylans among the 54 polysaccharides tested.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive tool kit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
If you publish research with this product, please let us know so we can cite your paper.
