Anti-Rhamnogalacturonan-I [CCRC-M2] Antibody (supernatant)
![Anti-Rhamnogalacturonan Ia [CCRC-M2] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1031_2001202001263110300.jpg "Anti-Rhamnogalacturonan-I [CCRC-M2] Antibody (supernatant)")
This mouse IgM monoclonal (CCRC-M2) was generated against Rhamnogalacturonan-I/MeBSA complex (Non-covalent) and belongs to the Rhamnogalacturonan-Ia clade of antibodies and binds to an epitope present in Rhamnogalacturonan-I from Gum Karaya, and sycamore and Arabidopsis pectic polysaccharides.
Highlights:
- Reacts with an epitope present in Ramnogalacturonan-I from Gum Karaya and sycamore and Arabidopsis pectic polysaccharides
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia
Part of The Investigator's Annexe program.
This mouse IgM monoclonal (CCRC-M2) was generated against Rhamnogalacturonan-I/MeBSA complex (Non-covalent) and belongs to the Rhamnogalacturonan-Ia clade of antibodies and binds to an epitope present in Rhamnogalacturonan-I from Gum Karaya, and sycamore and Arabidopsis pectic polysaccharides.
Highlights:
- Reacts with an epitope present in Ramnogalacturonan-I from Gum Karaya and sycamore and Arabidopsis pectic polysaccharides
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Accession ID: | CCRC-M2 |
| Antigen: | Rhamnogalacturonan I of Sycamore maple (Acer pseudoplatanus) |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | CCRC-M2 |
| Reactivity: | Sycamore, Arabidopsis, Gum Karaya |
| Immunogen: | Rhamnogalacturonan I/MeBSA complex (Non-covalent) |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant, Use undiluted or at 1:10 dilution |
| Tested Applications: | ELISA, Immunolabeling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
CCRC-M2 recognizes rhamnogalacturonan I from some plants, though the epitope remains uncharacterized.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
- G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
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