Anti-eEF3 Antibody
This rabbit polyclonal antibody was generated against N-terminal 6xHis-tagged full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 3 (eEF3).
Highlights
- Reacts with Saccharomyces cerevisiae eukaryotic translation elongation factor 3 (eEF3)
- Suitable for Western Blot applications
Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 3 is required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. eEF3 stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
This rabbit polyclonal antibody was generated against N-terminal 6xHis-tagged full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 3 (eEF3).
Highlights
- Reacts with Saccharomyces cerevisiae eukaryotic translation elongation factor 3 (eEF3)
- Suitable for Western Blot applications
Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 3 is required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. eEF3 stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
| Product Type: | Antibody |
| Antigen: | Saccharomyces cerevisiaeeukaryotic Translation Elongation Factor 3 (eEF3) |
| Accession ID: | P53978 , NP_014384 |
| Molecular Weight: | 116 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Saccharomyces cerevisiae, other fungi |
| Immunogen: | N-terminal 6xHis-tagged full length protein |
| Species Immunized: | Rabbit |
| Buffer: | Lyophilized whole serum |
| Tested Applications: | WB (1:10,000) |
| Storage: | 4C |
| Shipped: | Ambient temperature |
Protocol Notes
Reconstitute in 25uL water.
- Anand M, Chakraburtty K, Marton MJ, Hinnebusch AG, Kinzy TG. Functional interactions between yeast translation eukaryotic elongation factor (eEF) 1A and eEF3. J Biol Chem. 2003 Feb 28;278(9):6985-91. Epub 2002 Dec 18.
- Shamsuzzaman M, Bommakanti A, Zapinsky A, Rahman N, Pascual C, Lindahl L. Analysis of cell cycle parameters during the transition from unhindered growth to ribosomal and translational stress conditions. PLoS One. 2017 Oct 13;12(10):e0186494. View Article
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