Astrocyte LKB1 Knock Out Cell Line (ALKO)

Immortalized mouse astrocyte cell line (ALKO) with a stable knock out of liver kinase B1 (LKB1). Useful as a model for gliomas or for stem cell research.

Highlights:

  • Exhibit anchorage independent growth with doubling time of less than 24 hr
  • Express reduced levels of astrocyte marker GFAP (Glial fibrillary acidic protein)
  • Express stem cell markers including Sox2 and Oct4
  • Useful as a model for gliomas or for stem cell research

Liver kinase B1 (LKB1, also referred to as Serine/threonine-protein kinase STK11, Renal carcinoma antigen NY-REN-19) is a tumor suppressor serine/threonine-protein kinase that controls the activity of a large number of protein kinases including AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, and apoptosis.

From the laboratory of Douglas L. Feinstein, PhD, University of Illinois at Chicago.

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Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EB9001-FP
Astrocyte LKB1 Knock Out Cell Line (ALKO)
1 vial In stock
Regular Price:$695.00
On Sale:

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Specifications

Product Type: Cell Line
Name: ALKO (astrocyte LKB1 knock out)
Cell Type: Transformed mouse astrocyte
Accession ID: Q15831, CVCL_5I28
Source: Brain
Organism: Mouse (strain unknown)
Morphology: Fibroblast
Biosafety Level: BSL1
Subculturing: Standard trypsinization followed by 1:10 dilution
Growth Conditions: DMEM 10% FCS A/A
Cryopreservation: 10% DMSO in FBS
Storage: Liquid nitrogen
Shipped: Dry ice

Provider
From the laboratory of Douglas L. Feinstein, PhD, University of Illinois at Chicago.
Comments

Mouse ALKO cells (astrocyte LKB1 knock out) were prepared by treating astrocytes harboring a floxed version of the LKB1 gene with Adeno Associated Virus expressing the bacterial Cre-recombinase. After several passages, the resulting cells show anchorage independent growth, have a doubling time of less than 24 hr, lose expression of the astrocyte marker GFAP (Glial fibrillary acidic protein), and gain expression of stem cell markers including Sox2 and Oct4.

References

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