Anti-MuLV Transmembrane Protein [42/114] Antibody (supernatant)
![Anti-MuLV Transmembrane Protein [42/114] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1075_2001202001263310740.jpg "Anti-MuLV Transmembrane Protein [42/114] Antibody (supernatant)")
This mouse IgM monoclonal antibody [42/114] was generated against AKR K36 and AKR SL3 leukemia and is specific for rat murine leukemia virus (MuLV) transmembrane protein.
Highlights:
- Reacts with rat murine leukemia virus transmembrane protein
- Suitable for Western Blot and Immunoprecipitation applications
Murine leukemia viruses also known as MuLVs are retroviruses named for their ability to cause cancer in mouse hosts. They have multiple applications including gene therapy, cancer studies, as a model for retroviruses in viral clearance studies and also can used in biotechnology for reverse transcriptase.
From the laboratory of Abraham Pinter, PhD, Rutgers University.
Part of The Investigator's Annexe program.
This mouse IgM monoclonal antibody [42/114] was generated against AKR K36 and AKR SL3 leukemia and is specific for rat murine leukemia virus (MuLV) transmembrane protein.
Highlights:
- Reacts with rat murine leukemia virus transmembrane protein
- Suitable for Western Blot and Immunoprecipitation applications
Murine leukemia viruses also known as MuLVs are retroviruses named for their ability to cause cancer in mouse hosts. They have multiple applications including gene therapy, cancer studies, as a model for retroviruses in viral clearance studies and also can used in biotechnology for reverse transcriptase.
From the laboratory of Abraham Pinter, PhD, Rutgers University.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | Murine leukemia virus transmembrane protein |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | 42/114 |
| Reactivity: | Rat |
| Immunogen: | AKR K36 and AKR SL3 leukemia |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | WB, IP |
| Storage: | -80C |
| Shipped: | Cold Packs |
Notes
Original characterization of antibodies was done by ammonium sulphate precipitations followed by radioimmunoprecipitation assays.
Immunoprecipitation
Precipitation of intact virions with 44-114 antibody. Supernatants from NIH 3T3 cells infected with MuLV were immunoprecipitated in presence (+) and absence (-) of of NP-40 with 20u/mL of antibody. Precipitates were analyzed by SDS-Page and autoradiography. TM refers to the transmembrane subunit of Env. p15E and CA refers to the MuLV capsid protein, p30.
Adapted from: Virol. 2003 Apr;77(7):3993-4003.
- Lostrom, ME , Stone, MR, Tam M, Burnette WN, Pinter A, Nowinski RC. Monoclonal antibodies against murine leukemia viruses: identification of six antigenic determinants on the p15(E) and gp70 envelope proteins. Virology 1979 Oct 30;98(2):336-50.
- Hammerling U., Pinter A, and O'donnell PV, (1980) Monoclonal Antibodies as a tool in research on oncogenic viruses. p. 255-256. In G. A. Gerth (ed). The role of viruses in human cancer (Elsevier, Amsterdam).
- Pinter, A., Honnen, WJ, Tung, JS, O'Donnell, PV. Structural domains of endogenous murine leukemia virus gp70s containing specific antigenic determinants defined by monoclonal antibodies. Virology 1982 116(2):s 499-516
- Burkhart MD, Kayman SC, He Y, Pinter A. Distinct mechanisms of neutralization by monoclonal antibodies specific for sites in the N-terminal or C-terminal domain of murine leukemia virus SU. J Virol. 2003 Apr;77(7):3993-4003.
- Timilsina U, Umthong S, Lynch B, Stablewski A, Stavrou S. SERINC5 Potently Restricts Retrovirus Infection In Vivo. mBio. 2020;11(4):e00588-20. Published 2020 Jul 14. View article
- Umthong S, Lynch B, Timilsina U, Waxman B, Ivey EB, Stavrou S. Elucidating the Antiviral Mechanism of Different MARCH Factors. mBio. 2021 Mar 2;12(2):e03264-20. View article
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