Anti-MS4A4A [5C12] Antibody
![Anti-MS4A4A [5C12] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1092_2001202001263410910.jpg "Anti-MS4A4A [5C12] Antibody")
This mouse IgG1 monoclonal antibody [5C12] was generated against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4 (MS4A4A)-GFP and is specific for human extracellular A MS4A4A.
Highlights:
- Reacts with human MS4A4A
- Suitable for FACS and Immunoprecipitation applications
Recombinant versions available at our sister company, Absolute Antibody:
- Clone 5C12 available in various species, isotope and/or format
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 5C12
MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. CD20, the most well-known MS4A family member, is a B-cell-specific molecule associated with the B cell antigen receptor and functions in receptor-activated calcium influx. Several other MS4A family members are likely to be components of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
This mouse IgG1 monoclonal antibody [5C12] was generated against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4 (MS4A4A)-GFP and is specific for human extracellular A MS4A4A.
Highlights:
- Reacts with human MS4A4A
- Suitable for FACS and Immunoprecipitation applications
Recombinant versions available at our sister company, Absolute Antibody:
- Clone 5C12 available in various species, isotope and/or format
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 5C12
MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. CD20, the most well-known MS4A family member, is a B-cell-specific molecule associated with the B cell antigen receptor and functions in receptor-activated calcium influx. Several other MS4A family members are likely to be components of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
| Product Type: | Antibody |
| Antigen: | MS4A4A |
| Accession ID: | Q96JQ5 |
| Molecular Weight: | 26 kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 5C12 |
| Reactivity: | Human |
| Immunogen: | Mouse cell line 300-19 expressing transfected human MS4A4A-GFP |
| Species Immunized: | Mouse |
| Epitope: | Extracellular (distinct from clones 4H2 and 3F2) |
| Purification Method: | Protein G Affinity |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | FACS (0.5 ug/10^6 cells), IP (2ug) |
| Storage: | -20C |
| Shipped: | Cold packs |
Works best for FACS on primary cells using direct staining.
- Sanyal R, Polyak MJ, Zuccolo J, Puri M, Deng L, Roberts L, Zuba A, Storek J, Luider JM, Sundberg EM, Mansoor A, Baigorri E, Chu MP, Belch AR, Pilarski LM, Deans JP. MS4A4A: a novel cell surface marker for M2 macrophages and plasma cells. Immunol Cell Biol. 2017 Apr 11. doi: 10.1038/icb.2017.18.
If you publish research with this product, please let us know so we can cite your paper.
