Anti-CD20, C-Terminal [1440] Antibody
![Anti-CD20, N-Terminal [1440] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1093_2001202001263410920.jpg "Anti-CD20, C-Terminal [1440] Antibody")
This rabbit polyclonal antibody [1440] was generated against a synthetic peptide corresponding to residues 231-245 (SAEEKKEQTIEIKEE) of human CD20 fused to ovalbumin, and is specific for human CD20 (intracellular C-terminal region).
Highlights:
- Reacts with intracellular residues 231-245 of human CD20
- Valuable for both pre-clinical and clinical research applications
- Suitable for Western Blot and Immunoprecipitation applications
CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
Part of The Investigator's Annexe program.
This rabbit polyclonal antibody [1440] was generated against a synthetic peptide corresponding to residues 231-245 (SAEEKKEQTIEIKEE) of human CD20 fused to ovalbumin, and is specific for human CD20 (intracellular C-terminal region).
Highlights:
- Reacts with intracellular residues 231-245 of human CD20
- Valuable for both pre-clinical and clinical research applications
- Suitable for Western Blot and Immunoprecipitation applications
CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | CD20 (CD20-C) |
| Accession ID: | P11836 |
| Molecular Weight: | 33-35 kDa |
| Clonality: | Polyclonal |
| Clone Name: | 1440 |
| Reactivity: | Human |
| Immunogen: | Peptide conjugated to ovalbumin |
| Species Immunized: | Rabbit |
| Epitope: | C-terminal residues 231-245; SAEEKKEQTIEIKEE |
| Buffer: | Serum |
| Tested Applications: | WB (1:2,000) |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Western Blot of CD20 N and C-terminus
Western blots of human Ramos B-cells lysates probed with rabbit anti-CD20N (targeting the N-terminal residues 25-41) and rabbit anti-CD20C (targeting the C-terminal residues 231-245), pre-incubated with immunizing peptide (lanes 2 and 5), irrelevant peptide (lanes 3 and 6) or no peptide (lanes 1 and 4).
Adapted from: Polyak MJ, et al. J Immunol. 1998 Oct 1;161(7):3242-8.
- Polyak MJ, Tailor SH, Deans JP. Identification of a cytoplasmic region of CD20 required for its redistribution to a detergent-insoluble membrane compartment. J Immunol. 1998 Oct 1;161(7):3242-8.
- Deans JP, Robbins SM, Polyak MJ, Savage JA. Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem. 1998 Jan 2;273(1):344-8.
- Popoff IJ, Savage JA, Blake J, Johnson P, Deans JP. The association between CD20 and Src-family Tyrosine kinases requires an additional factor. Mol Immunol. 1998 Mar;35(4):207-14.
- Polyak MJ, Deans JP. Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62.
- Polyak MJ, Li H, Shariat N, Deans JP. CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins. J Biol Chem. 2008 Jul 4;283(27):18545-52.
If you publish research with this product, please let us know so we can cite your paper.
