JHE is a canine hemangiosarcoma cell line derived from a tumor in the heart of a 12-year old neutered male Golden Retriever with disseminated, metastatic disease. It grows in vitro.
Hemangiosarcoma is a rapidly growing, highly invasive variety of cancer, occurring commonly in dogs. It is a sarcoma arising from the lining of blood vessels. A frequent cause of death is the rupturing of this tumor, causing the patient to rapidly bleed to death.
From the laboratory of Jaime F. Modiano, VMD, PhD, University Minnesota, Twin Cities.
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Product Type: | Cell Line |
Name: | JHE |
Cell Type: | Hemangiosarcoma |
Accession ID: | CVCL_5I40 |
Organism: | Dog |
Source: | Heart |
Morphology: | Spindle |
Biosafety Level: | 2 |
Subculturing: | Split 1:2-1:4 when cells reach ~80% confluence |
Growth Conditions: | Ham's F12, 10% FBS, 0.05mg/ml ECGS, 0.01mg/ml heparin, 10mM HEPES Buffer, 100ug/ml Primocin |
Cryopreservation: | 90%FBS/10% DMSO |
Mycoplasma Tested: | Yes |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
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