L-Azidohomoalanine, HCI salt
L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis.
Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis.
Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Specifications
| Product Type: | Small Molecule |
| Chemical Formula: | C4H9ClN4O2 |
| CAS number: | 942518-29-8 |
| Molecular Weight: | 180.59 (HCl salt) |
| Variant MPN: | 1066 |
| Format: | White crystalline |
| Purity: | >95% (H NMR) |
| Solubility: | Water, DMSO, DMF |
| Storage: | -20C |
| Shipped: | Ambient |
References
- Bertalovitz AC, Osterbur Badhey ML, McDonald TV. Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel. J Biol Chem. 2018 Jun 15. View Article
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