Actin Force Sensor (cpstFRET) Expressing Cell Lines
These cell lines express actin force probes, allowing for the measurement of forces of actin and actinin in living cells via simple anisotropy FRET ratio R to measure changes in FRET.
Highlights:
- Integrate naturally into cellular microfilaments
- Report force in actin and the actin cross-linker actinin
- Available as immortalized HEK293 or MDCK cell lines
Available Versions:
- AcpA - One actin molecule attached on each end of cpstFRET
- cpA - One actin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension. (force-free control)
- M-cpstFRET - Actinin with the sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin
- C-cpstFRET - C-terminal–tagged actinin (force-free control)
Mechanical signaling plays many roles in cell physiology, including stem cell differentiation and reprogramming. The actin force probe, named actin–cpstFRET (circularly permuted stretch sensitive FRET)–actin (AcpA), consists of a dipole orientation-based FRET sensor called cpstFRET flanked with β-actin monomers. The sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs. Once expressed in cells, the actin probes incorporate into mainly into f-actin filaments and allow for the measurement of forces of actin and actin in in living cells via simple anisotropy FRET ratio R to measure changes in FRET.
From the laboratory of Frederick Sachs, PhD, University at Buffalo.
These cell lines express actin force probes, allowing for the measurement of forces of actin and actinin in living cells via simple anisotropy FRET ratio R to measure changes in FRET.
Highlights:
- Integrate naturally into cellular microfilaments
- Report force in actin and the actin cross-linker actinin
- Available as immortalized HEK293 or MDCK cell lines
Available Versions:
- AcpA - One actin molecule attached on each end of cpstFRET
- cpA - One actin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension. (force-free control)
- M-cpstFRET - Actinin with the sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin
- C-cpstFRET - C-terminal–tagged actinin (force-free control)
Mechanical signaling plays many roles in cell physiology, including stem cell differentiation and reprogramming. The actin force probe, named actin–cpstFRET (circularly permuted stretch sensitive FRET)–actin (AcpA), consists of a dipole orientation-based FRET sensor called cpstFRET flanked with β-actin monomers. The sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs. Once expressed in cells, the actin probes incorporate into mainly into f-actin filaments and allow for the measurement of forces of actin and actin in in living cells via simple anisotropy FRET ratio R to measure changes in FRET.
From the laboratory of Frederick Sachs, PhD, University at Buffalo.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Cell Line |
| Name: | Beta-M-HEK |
| Cell Type: | HEK-293, Human Embryonic Kidney |
| Accession ID: | O43707, Q08043, P12814, P35609 |
| Organism: | Homo sapiens,Human, fetus |
| Morphology: | Epithelial; grow as adherent monolayer |
| Source: | Human Embryonic Kidney |
| Biosafety Level: | 2 (contains adenovirus) |
| Subculturing: | A subcultivation ratio of 1:2 to 1:6 is recommended. Replace medium every 2 to 3 days |
| Growth Conditions: | DMEM w High Glucose + 10% Fetal Bovine Serum + Penn/strep |
| Cryopreservation: | Complete growth medium, 95%; DMSO, 5% |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
Actin Force Sensor (cpstFRET) Expressing Cell Line Protocol
Actin Force Sensor (cpstFRET) Expressing Cell Lines
| Cells: | AcpA HEK293 | cpA HEK293 | M-cpstFRET HEK293 | C-cpstFRET HEK293 | M-cpstFRET MDCK | C-cpstFRET MDCK |
| Cell Type: | HEK-293, Human Embryonic Kidney | HEK-293, Human Embryonic Kidney | HEK-293, Human Embryonic Kidney | HEK-293, Human Embryonic Kidney | MDCK | MDCK |
| Organism: | Homo sapiens, Human, fetus | Homo sapiens, Human, fetus | Homo sapiens, Human, fetus | Homo sapiens, Human, fetus | Dog, Canis familiaris | Dog, Canis familiaris |
| Morphology: | Epithelial | |||||
| Source Organ/Tissue: | Human Embryonic Kidney | Human Embryonic Kidney | Human Embryonic Kidney | Human Embryonic Kidney | Normal Adult Kidney, Female | Normal Adult Kidney, Female |
| Cell Line Name: | Beta-M-HEK | Beta-C-HEK | Actinin-M-cpstFRET | Actinin-C-cpstFRET | Actinin-M-cpstFRET | Actinin-C-cpstFRET |
| Biosafety Level: | 2 (contains adenovirus) | 2 (contains adenovirus) | 2 (contains adenovirus) | 2 (contains adenovirus) | 1 | 1 |
| Growth Properties: | Adherent monolayer | |||||
| Growth Medium: | DMEM w High Glucose + 10% Fetal Bovine Serum + Penn/strep | |||||
| Sub-culturing: | A subcultivation ratio of 1:2 to 1:6 is recommended. Replace medium every 2 to 3 days | |||||
| Cryopersavation: | Complete growth medium, 95%; DMSO, 5% | |||||
| Storage Temperature: | Liquid Nitrogen | |||||
| Shipped: | Dry Ice | |||||
- Guo, J., Wang, Y., Sachs, F. & Meng, F. Actin stress in cell reprogramming. Proceedings of the National Academy of Sciences of the United States of America 111, E5252-5261 (2014).
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