Anti-Beta-Actin Antibody
This rabbit IgG polyclonal antibody was generated against smooth muscle actin and filamin and is specific for human actin, beta-actin.
Highlights:
- Reacts with human actin, beta-actin
- Recommended for Western Blot applications
Beta-actin is a highly conserved protein that are involved in cell motility, structure, and integrity. Beta- actin, is one of the six isoforms in humans, and one of the two nonmuscle cytoskeletal actins. Beta-actin is commonly used as a loading control.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
This rabbit IgG polyclonal antibody was generated against smooth muscle actin and filamin and is specific for human actin, beta-actin.
Highlights:
- Reacts with human actin, beta-actin
- Recommended for Western Blot applications
Beta-actin is a highly conserved protein that are involved in cell motility, structure, and integrity. Beta- actin, is one of the six isoforms in humans, and one of the two nonmuscle cytoskeletal actins. Beta-actin is commonly used as a loading control.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
| Product Type: | Antibody |
| Antigen: | Human Actin, beta-actin |
| Accession ID: | P60709 |
| Molecular Weight: | 42 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Human |
| Immunogen: | Smooth muscle actin and filamin |
| Species Immunized: | Rabbit |
| Purification Method: | Rabbit serum was affinity purified to separate only the antibody that binds to actin only |
| Tested Applications: | Western Blot (1:2,000; 1min exposure) |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Western Blot
Lane 1, Human Red Cell Membrane; Lane 2, Human Melanoma (1205 LU); Lane 3, Human Ovarian Cancer (RMG1); Lane 4, Human Ovarian Cancer (OVCAR3); Lane 5, Human Ovarian Cancer (OV90). Gel loads: Lane 1, 5mg; Lanes 2-5, 25mg. Antibody dilution: 1:2,000, 1min exposure.
- Hock RS, Davis G, Speicher DW. Purification of human smooth muscle filamin and characterization of structural domains and functional sites. Biochemistry. 1990 Oct 9;29(40):9441-51.
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