This mouse IgG monoclonal antibody was generated against human red blood cell a-spectrin N terminal 1/3rd and is specific for human red blood cell a-spectrin N terminal 1/3rd of the protein.
Highlights:
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Specifically, Alpha-spectrin is a tetramer and mutations in alpha-spectrin can result in a variety of hereditary red blood cell disorders (e.g. spherocytic hemolytic anemia including elliptocytosis type 2, pyropoikilocytosis).
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
Product Type: | Antibody |
Alternative Name(s): | Erythroid alpha-spectrin,Spectrin alpha chain, erythrocytic 1 |
Antigen: | Human Red Blood Cell a-Spectrin N terminal 1/3rd of the protein |
Accession ID: | P02549 |
Molecular Weight: | 280 kDa |
Isotype: | IgG |
Clonality: | Monoclonal |
Clone Name: | 9.5.21 |
Reactivity: | Human |
Immunogen: | Human Red Blood Cell a-Spectrin N terminal 1/3rd of the protein |
Species Immunized: | Mouse |
Purification Method: | Affinity Purified |
Tested Applications: | Western Blot (1:250,000; 30sec exposure) |
Storage: | -20C |
Shipped: | Cold packs |
Western Blot
Lane 1, Human Melanoma (1205 LU); Lane 2, Human Ovarian Cancer (RMG1); Lane 3, Human Ovarian Cancer (OV90); Lane 4, Human Red Cell Membrane. Gel loads: Lanes 1-3, 25mg; Lane 4, 5mg. Antibody dilution: 1:250,000, 30sec exposure.
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