Anti-E2F6 [LLF6-2] Antibody
This mouse monoclonal antibody was generated against human E2F6 (full length, 6X His-tagged) and recognizes both human and mouse E2F6.
E2F6 is a winged helix transcription factor important for regulating the expression of genes encoding proteins required for meiosis, cell cycle exit and skeletal patterning. E2F6 also interacts with members of the Bmi1 containing polycomb group complex and predominantly functions as a repressor of transcription.
From a laboratory at Massachusetts Institute of Technology.
Part of The Investigator's Annexe program.
This mouse monoclonal antibody was generated against human E2F6 (full length, 6X His-tagged) and recognizes both human and mouse E2F6.
E2F6 is a winged helix transcription factor important for regulating the expression of genes encoding proteins required for meiosis, cell cycle exit and skeletal patterning. E2F6 also interacts with members of the Bmi1 containing polycomb group complex and predominantly functions as a repressor of transcription.
From a laboratory at Massachusetts Institute of Technology.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Name: | E2F6 monoclonal antibody |
| Accession ID: | O54917 |
| Host: | mouse |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: | LLF6-2 |
| Specificity: | human, mouse |
| Immunogen: | Antibody was raised against 6x His-tagged full length human E2F6, purified from bacteria |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Tested Applications: | ELISA, Western Blot, Immunoprecipitation, Gel Supershift Assay |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | +4C or -20C (avoid repeated freeze / thaw cycles) |
| Shipped: | Cold packs |
Immunoprecipitation
Human ML-1 cell lysates were immunoprecipitated with control, E2F6 (from LLF6-1), or RYBP antibodies and resolved by SDS/PAGE, then probed by E2F6 (from LLF6-2) antibody.
- Courel M, Friesenhahn LB, Lees JA (2008) E2f6 an Bmi1 cooperate in axial skeletal development. Dev Dyn. (237): 1232-1242
- Trimarchi JM, Fairchild B, Wen J, Lees JA (2001) The E2F6 transcription factor is a component of the mammalian Bmi1-containing polycomb complex. Proc. Natl. Acad. Sci. USA 98(4): 1519 - 24
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