Anti-VSV-N [10G4] Antibody
This monoclonal antibody reacts with VSV N protein.
Highlights:
- Reacts with VSV-N protein
- Suitable for Westerm Blot applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 10G4
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The N protein (or nucleocapsid protein) tightly binds viral RNA. This activity of the N protein protects the viral genome from cellular sensors and nuclease degradation. Additionally, accumulation of the N protein during VSV replication results in a switch from viral gene transcription to genome replication.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
This monoclonal antibody reacts with VSV N protein.
Highlights:
- Reacts with VSV-N protein
- Suitable for Westerm Blot applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 10G4
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The N protein (or nucleocapsid protein) tightly binds viral RNA. This activity of the N protein protects the viral genome from cellular sensors and nuclease degradation. Additionally, accumulation of the N protein during VSV replication results in a switch from viral gene transcription to genome replication.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Antibody |
| Name: | Anti-VSV-N [10G4] |
| Antigen: | VSV-N |
| Accession ID: | P03521 |
| Host: | Mouse |
| Isotype: |
EB0009: IgG2a kappa Recombinant versions: see product name |
| Clonality: | Monoclonal |
| Clone Name: | 10G4 |
| Specificity: | VSV-Ind nucleocapsid (N) protein |
| Reactivity: | Human |
| Immunogen: | VSV infection |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Buffer: |
EB0009: PBS, 0.05% (w/v) Sodium Azide Recombinant versions: PBS with 0.02% Proclin 300 |
| Tested Applications: | Western blot (1:1000) |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | -20C (avoid repeated freeze / thaw cycles) |
| Shipped: | Cold packs |
Provider
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
References
- Lyles DS, Puddington L, McCreedy BJ Jr. Vesicular stomatitis virus M protein in the nuclei of infected cells. J Virol. 1988 Nov;62(11):4387-92. PubMed PMID: 2845149
- Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982.
- Malikov V, da Silva ES, Jovasevic V, Bennett G, de Souza Aranha Vieira DA, Schulte B, Diaz-Griffero F, Walsh D, Naghavi MH. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun. 2015 Mar 30;6:6660. doi: 10.1038/ncomms7660. PubMed PMID: 25818806; PubMed Central PMCID: PMC4380233. View Article
- Bresk CA, Hofer T, Wilmschen S, Krismer M, Beierfuß A, Effantin G, Weissenhorn W, Hogan MJ, Jordan APO, Gelman RS, Montefiori DC, Liao HX, Schmitz JE, Haynes BF, von Laer D, Kimpel J. Induction of Tier 1 HIV Neutralizing Antibodies by Envelope Trimers Incorporated into a Replication Competent Vesicular Stomatitis Virus Vector. Viruses. 2019 Feb 15;11(2). pii: E159. View Article
- Hulswit RJG, Lang Y, Bakkers MJG, et al. Human coronaviruses OC43 and HKU1 bind to 9-O-acetylated sialic acids via a conserved receptor-binding site in spike protein domain A. Proc Natl Acad Sci U S A. 2019;116(7):2681–2690. View Article
- Tortorici MA, Walls AC, Lang Y, Wang C, Li Z, Koerhuis D, Boons GJ, Bosch BJ, Rey FA, de Groot RJ, Veesler D. Structural basis for human coronavirus attachment to sialic acid receptors. Nat Struct Mol Biol. 2019 Jun;26(6):481-489. View Article
- Schreiber LM, Urbiola C, Das K, et al. The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer. Br J Cancer. 2019;121(8):647-658. View article
- Patil G, Xu L, Wu Y, Song K, Hao W, Hua F, Wang L, Li S. TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Vesicular Stomatitis Virus Infection. Viruses. 2020 Jan 22;12(2):131. View article
- Duehr J, McMahon M, Williamson B, Amanat F, Durbin A, Hawman DW, Noack D, Uhl S, Tan GS, Feldmann H, Krammer F. Neutralizing Monoclonal Antibodies against the Gn and the Gc of the Andes Virus Glycoprotein Spike Complex Protect from Virus Challenge in a Preclinical Hamster Model. mBio. 2020 Mar 24;11(2):e00028-20. View article
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