RS-12 - Large Stokes-Shift NIR Dye (653,676 Ex/805 Em)-Acid
RS-12 is a near-infrared fluorescent dye with an excitation of 653-676nm and emission of 805nm.
Highlights:
- pH sensitive lactone ring opening induced spectral change near pH 7.4, pH sensitive hydroxyl ionization induced spectral change > pH 9
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
RS-12 is a near-infrared fluorescent dye with an excitation of 653-676nm and emission of 805nm.
Highlights:
- pH sensitive lactone ring opening induced spectral change near pH 7.4, pH sensitive hydroxyl ionization induced spectral change > pH 9
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
| Product Type: | Small Molecule |
| Name: | 2-(13-hydroxy-1-oxo-1H-dibenzo[c,h]xanthen-7-yl)benzoic acid or 1,13- dihydroxy-Spiro[7H-dibenzo[c,h] xanthene-7,1'(3'H)- isobenzofuran]-3'-one |
| CAS number: | 1380077-16-6 |
| Chemical Formula: | C28H16O5 |
| Extinction Coefficient: | 7,910 in pH 9, 7,564 in pH 12 |
| Molecular Weight: | 432.42 |
| Tested Applications: | NIR chromophore, potential solar cell application, model compound exhibiting low barrier hydrogen bond, pH sensitive lactone ring opening induced spectral change near pH 7.4, pH sensitive hydroxyl ionization induced spectral change > pH 9. |
| Ex/Em Maxima: | Emission at 805 in pH 9 |
| Abs Max (nm): | 653 in pH 9, 676 in pH 12 |
| Detection Method: | Fluorescence |
| Storage: | -20C; protect from light |
| Shipped: | Cold packs |
- Sibrian-Vazquez M, Escobedo JO, Lowry M, Strongin RM. Progress toward red and near-infrared (NIR) emitting saccharide sensors. Pure Appl Chem. 2012 Nov 1;84(11):2443-2456.
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