RS-13 - Large Stokes-Shift NIR Dye (495-590 Ex/590-750 Em)-Acid
RS-13 is a near-infrared fluorescent dye with an excitation of 495-590nm and emission of 590-750nm.
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
RS-13 is a near-infrared fluorescent dye with an excitation of 495-590nm and emission of 590-750nm.
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
| Product Type: | Small Molecule |
| Name: | 1,10-dihydroxy-Spiro[7H-benzo[c]xanthene-7,1'(3'H)-isobenzofuran]-3'-one |
| Chemical Formula: | C24H14O5 |
| CAS number: | 1380077-20-2 |
| Molecular Weight: | 382.36 |
| Extinction Coefficient: | 16,670 in pH 6, 17,182 in pH 12 |
| Tested Applications: | pH sensitive red or NIR emission |
| Ex/Em Maxima: | 590 in pH 6, 750 in pH 12 12 |
| Abs Max (nm): | 495, 525 in pH 6, 590 in pH 12 |
| Detection Method: | Fluorescence |
| Storage: | -20C; protect from light |
| Shipped: | Cold packs |
- Sibrian-Vazquez M, Escobedo JO, Lowry M, Strongin RM. Progress toward red and near-infrared (NIR) emitting saccharide sensors. Pure Appl Chem. 2012 Nov 1;84(11):2443-2456.
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