RS-15 - Large Stokes-Shift NIR Dye (495 Ex/580 Em)-Acid
RS-15 is a near-infrared fluorescent dye with an excitation of 495nm and emission of 580nm.
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
RS-15 is a near-infrared fluorescent dye with an excitation of 495nm and emission of 580nm.
The distance between the excitation and emission wavelengths is called the Stokes Shift and is a key aspect in the detection of the emitted fluorescence in biological applications. The detection of emitted fluorescence can be difficult to distinguish from the excitation light when using fluorophores with very small Stokes shifts, because the excitation and emission wavelengths greatly overlap. Conversely, fluorophores with large Stokes shifts are easy to distinguish because of the large separation between the excitation and emission wavelengths. The Stokes shift is especially critical in multiplex fluorescence applications, because the emission wavelength of one fluor may overlap, and therefore excite, another fluor in the same sample.
From the laboratory of Robert M. Strongin, PhD, Portland State University.
| Product Type: | Small Molecule |
| Name: | 2-(1-methoxy- 10-oxo-10H-benzo[c] xanthen-7-yl)-benzoic acid, methyl ester |
| Chemical Formula: | C26H18O5 |
| CAS number: | 1380077-22-4 |
| Molecular Weight: | 410.42 |
| Extinction Coefficient: | 15,1610 in pH 7.4 |
| Tested Applications: | pH independent red emission |
| Ex/Em Maxima: | 580 in pH 7.4 |
| Abs Max (nm): | 495, 530 in pH 7.4 |
| Detection Method: | Fluorescence |
| Storage: | -20C; protect from light |
| Shipped: | Cold packs |
- Sibrian-Vazquez M, Escobedo JO, Lowry M, Strongin RM. Progress toward red and near-infrared (NIR) emitting saccharide sensors. Pure Appl Chem. 2012 Nov 1;84(11):2443-2456.
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