This mouse monoclonal antibody was generated against a mammalian expressed cohesin-hAMIGO2 fusion protein and recognizes human Amphoterin-induced protein 2 (AMIGO2).
Highlights:
Amphoterin-induced protein 2 (AMIGO2), also known as Alivin1, contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. AMIGO2 may contribute to signal transduction through its intracellular domain. AMIGO2 may be required for tumorigenesis of a subset of gastric adenocarcinomas.
From the laboratory of Gerard Zurawski, PhD, Baylor Scott & White Research Institute.
Product Type: | Antibody |
Antigen: | cohesin-Amigo2 Ecto domain |
Accession ID: | NP_862830 |
Molecular Weight: | 60,374 Da (fusion protein) Ecto domain of Amigo2 (40-397) |
Isotype: | IgG2a |
Clonality: | Monoclonal |
Clone Name: | AB60_71.30G9.1G2 |
Reactivity: | Human |
Immunogen: | Mammalian expressed C-terminal fusion protein. N-terminal HisTag-cohesin-hAmigo2 (residues 40-397) |
Species Immunized: | mouse BalbC (myeloma fusion with SP2-0/Ag14) |
Purification Method: | MabSelect (ProA Affinity) |
Buffer: | PBS (MgCa++) |
Tested Applications: | ELISA, FACS, IHC |
Storage: | Long term -80C; Short term 4C; avoid freeze thaws |
Shipped: | Cold packs |
Immunohistochemistry
Immunohistochemistry of paraffin-embedded skin utilizing Anti-hAMIGO2 30G9.1G2. (A) Representative images of IHC performed on paraffin-embeded skin. (B) Isotype control shows clear contrast to actual staining pattern with AMIGO2 antibody.
FACS
(A) Anti-hAMIGO2 30G9.1G2 on CHO cells stably expressing full length AMIGO2. (B) Anti-hAMIGO2 30G9.1G2 on CHO cells. Purified Mabs stained at 2 ug/ml; 500000 cells/pt, Secondary staining with 1:250 goat anti-mouse IgG PE (BD Biosciences); PBS 2% BSA staining buffer used.
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