GPR55 Expressing HEK293 Cell Lines
Human and mouse G protein-coupled receptor 55 (GPR55) expressing HEK293 cells.
GPR55, along with GPR119 and GPR18, have been implicated as possible novel cannabinoid receptors. Two endogenous ligands, lysophosphatidyl inositol and lysophosphatidyl glucoside, for GPR55 have been identified. Ongoing research has implicated GPR55 in a variety of normal and pathological processes, including cancer, axon guidance, synaptic transmission, immune function, and metabolic processes.
From the laboratory of Ken Mackie, MD, Indiana University.
Human and mouse G protein-coupled receptor 55 (GPR55) expressing HEK293 cells.
GPR55, along with GPR119 and GPR18, have been implicated as possible novel cannabinoid receptors. Two endogenous ligands, lysophosphatidyl inositol and lysophosphatidyl glucoside, for GPR55 have been identified. Ongoing research has implicated GPR55 in a variety of normal and pathological processes, including cancer, axon guidance, synaptic transmission, immune function, and metabolic processes.
From the laboratory of Ken Mackie, MD, Indiana University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Cell Line |
| Cell Type: | HEK293 |
| Accession ID: | Q9Y2T6 |
| Organism: | Mouse |
| Source: | Para-kidney tumor, adenovirus-transformed |
| Morphology: | Multi-polar and variable, adherent |
| Biosafety Level: | BSL-2 |
| Subculturing: | 0.05% Trypsin-EDTA/Versene |
| Growth Conditions: |
DMEM, 10% FBS, (400-1000 microgram/mL) G418, Pen/Strep (See  Suggested Protocol & "Comments" section for details)
|
| Cryopreservation: | 90% FBS, 10% DMSO |
| Comments: | Fast growing, adherent cell line, little contact inhibition. It is best to keep the cells under selection with G418, but the GPR55 lines should be stable without selection for several passages. The amount of G418 depends on the lot and manufacturer. We would use the lowest concentration that kills HEK cells you have in house (usually 400-1000 microgram/mL). |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
Documentation
Provider
From the laboratory of Ken Mackie, MD, Indiana University.
References
- Lauckner JE, Jensen JB, Chen HY, Lu HC, Hille B, Mackie K. GPR55 is a cannabinoid receptor that increases intracellular calcium and inhibits M current. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2699-704. doi: 10.1073/pnas.0711278105.
- Hill JD, Zuluaga-Ramirez V, Gajghate S, Winfield M, Persidsky Y. Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis. Br J Pharmacol. 2018 Jun 11. View Article
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