These mouse IgG1 monoclonal antibodies were generated against protein and recognize A. thaliana, Brassica and other closely related eukaryotes Actin-related protein 8 (ARP8).
Highlights:
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Product Type: | Antibody |
Alternative Name(s): | INO80 complex subunit N |
Antigen: | Actin-related protein 8 (ARP8), including recombinant forms |
Accession ID: | Q9H981 |
Molecular Weight: | 52 kDa |
Isotype: | IgG1 |
Clonality: | Monoclonal |
Clone Name: | 13F10.A4.G6 or 13F10.A4.G7 |
Reactivity: | A. thaliana, Brassica and other eukaryotes for which ARP8 is closely related to thaliana's |
Immunogen: | Protein |
Species Immunized: | Mouse |
Epitope: |
N-Terminal (clone 13F10): aa 2-ILKKVWG SVWNRSNSGKDLVNHQRA-26 C-Terminal (clone 12F5): aa 447-SNLSIFPGPWCITRKQFRRKSRLMW-471 |
Purification Method: | Not purified, available as antibody supernatant |
Buffer: | Cell Culture Supernatant |
Tested Applications: | WB, ELISA, IF, IB, Immunocytochemistry |
Storage: | -80C |
Shipped: | Cold Packs |
MAbARP8-N reacted with the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli and endogenous ARP8 of identical molecular weight in Arabidopsis plant extracts.. MAbARP8-N detected ARP8 protein in all vegetative and reproductive organs examined including seedlings, roots and siliques, although higher concentrations were observed in developing flower buds and flowers within the inflorescence
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