Anti-Argonaute-2 (AGO2) [4F9] Antibody
![Anti-Argonaute-2 (AGO2) [4F9] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1423_2001202001264714220.jpg "Anti-Argonaute-2 (AGO2) [4F9] Antibody")
This mouse IgG1 monoclonal antibody was generated from a BALB/cJ mouse primed with pristane and immunized with full-length soluble recombinant human Argonaute-2 (Ago2). This antibody is able to immunoprecipitate human Ago2, as well as Ago1, Ago3, and Ago4 (reference 1).
Highlights:
- Recognizes human Ago2, but also Ago1, Ago3, and Ago4
- Most suitable for immunoprecipitation and ELISA applications but also used in special application in immunofluorescence
- Demonstrated use in immunoprecipitation of microRNAs that are associated with Ago2 (reference 1 and 2)
Argonaute (Ago) proteins are the key components that binds siRNA and micrRNA in the RNA induced silencing complex (RISC) complex with molecular masses of approximately 100 kDa and containing PAZ and PIWI domains. Four of these, Ago1 to Ago4, have been demonstrated to associate with miRNAs in humans, however, only Ago2 has been demonstrated to possess the activity of siRNA-guided mRNA cleavage.
From the laboratory of Edward K. Chan, PhD, University of Florida.
This mouse IgG1 monoclonal antibody was generated from a BALB/cJ mouse primed with pristane and immunized with full-length soluble recombinant human Argonaute-2 (Ago2). This antibody is able to immunoprecipitate human Ago2, as well as Ago1, Ago3, and Ago4 (reference 1).
Highlights:
- Recognizes human Ago2, but also Ago1, Ago3, and Ago4
- Most suitable for immunoprecipitation and ELISA applications but also used in special application in immunofluorescence
- Demonstrated use in immunoprecipitation of microRNAs that are associated with Ago2 (reference 1 and 2)
Argonaute (Ago) proteins are the key components that binds siRNA and micrRNA in the RNA induced silencing complex (RISC) complex with molecular masses of approximately 100 kDa and containing PAZ and PIWI domains. Four of these, Ago1 to Ago4, have been demonstrated to associate with miRNAs in humans, however, only Ago2 has been demonstrated to possess the activity of siRNA-guided mRNA cleavage.
From the laboratory of Edward K. Chan, PhD, University of Florida.
| Product Type: | Antibody |
| Antigen: | Human Argonaute-2 |
| Accession ID: | Q9UKV8 |
| Molecular Weight: | 100 kDa |
| Isotype: | IgAk |
| Clonality: | Monoclonal |
| Clone Name: | 4F9 |
| Reactivity: | Human |
| Immunogen: | Pristane-priming and full length soluble recombinant human Ago2 |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | ELISA, IF, IP |
| Storage: | -20C |
| Shipped: | Cold Packs |
- Ikeda K, Satoh M, Pauley KM, Fritzler MJ, Reeves WH, Chan EK. Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody. J Immunol Methods. 2006 Dec 20;317(1-2):38-44.
- Hendrickson DG, Hogan DJ, McCullough HL, Myers JW, Herschlag D, Ferrell JE, Brown PO. Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA. PLoS Biol. 2009 Nov;7(11):e1000238.
- Sheng P, Fields C, Aadland K, Wei T, Kolaczkowski O, Gu T, Kolaczkowski B, Xie M. Dicer cleaves 5'-extended microRNA precursors originating from RNA polymerase II transcription start sites. Nucleic Acids Res. 2018;46:5737-52. PMC:6009592.
- Fields CJ, Li L, Hiers NM, Li T, Sheng P, Huda T, Shan J, Gay L, Gu T, Bian J, Kilberg MS, Renne R, Xie M. Sequencing of Argonaute-bound microRNA/mRNA hybrids reveals regulation of the unfolded protein response by microRNA-320a. PLoS Genet. 2021 Dec 16;17(12):e1009934. doi: 10.1371/journal.pgen.1009934. PMID: 34914716; PMCID: PMC8675727.
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