Anti-SUMO2/SUMO3 [8A2] Antibody
![Anti-SUMO2/SUMO3 [8A2] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1461_2001202001264814600.jpg "Anti-SUMO2/SUMO3 [8A2] Antibody")
This mouse IgG monoclonal antibody was generated against recombinant protein and recognizes SUMO2 and SUMO3 in all vertebrates tested.
Highlights:
- Reacts with vertebrate SUMO2 and SUMO 3
- Does not react with SUMO 1
- Suitable for Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot applications
Small ubiquitin-related modifiers (SUMOs) are posttranslationally and covalently conjugated to hundreds of other proteins, thereby regulating a wide range of cellular functions, including transcription, DNA replication and repair, nucleocytoplasmic transport, chromosome segregation, mitochondrial fission and ion channel gating.
Whereas invertebrates express only one SUMO, vertebrates express three: SUMO1, SUMO2 and SUMO3. SUMO2 and SUMO3 are ~95% identical to each other (and thus often referred to as SUMO2/3), but they are only ~45% identical to SUMO1. SUMO2/3 also more readily form polymeric chains compared to SUMO1, due to the presence of a consensus sumoylation site near their N-termini. These factors suggest that SUMO2/3 may have targets and functions distinct from SUMO1.
From the laboratory of Michael J. Matunis, PhD, The Johns Hopkins University.
This mouse IgG monoclonal antibody was generated against recombinant protein and recognizes SUMO2 and SUMO3 in all vertebrates tested.
Highlights:
- Reacts with vertebrate SUMO2 and SUMO 3
- Does not react with SUMO 1
- Suitable for Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot applications
Small ubiquitin-related modifiers (SUMOs) are posttranslationally and covalently conjugated to hundreds of other proteins, thereby regulating a wide range of cellular functions, including transcription, DNA replication and repair, nucleocytoplasmic transport, chromosome segregation, mitochondrial fission and ion channel gating. Whereas invertebrates express only one SUMO, vertebrates express three: SUMO1, SUMO2 and SUMO3. SUMO2 and SUMO3 are ~95% identical to each other (and thus often referred to as SUMO2/3), but they are only ~45% identical to SUMO1. SUMO2/3 also more readily form polymeric chains compared to SUMO1, due to the presence of a consensus sumoylation site near their N-termini. These factors suggest that SUMO2/3 may have targets and functions distinct from SUMO1.
From the laboratory of Michael J. Matunis, PhD, The Johns Hopkins University.
| Product Type: | Antibody |
| Antigen: | SUMO2 and SUMO3 |
| Accession ID: | P61956, P55854 |
| Host: | Mouse |
| Molecular Weight: | 12kDa |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Reactivity: | All vertebrates tested |
| Immunogen: | Recombinant protein |
| Purification Method: | Protein A/G |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB, IF, IHC, IP |
| Storage: | -20C |
| Shipped: | Cold Packs |
Representative (A) Western Blot and (B) Immunofluorescence data using HeLa whole cell lysates.
- Zhang XD, Goeres J, Zhang H, Yen TJ, Porter AC, Matunis MJ. SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Mol Cell. 2008 Mar 28;29(6):729-41. doi: 10.1016/j.molcel.2008.01.013. PubMed PMID: 18374647; PubMed Central PMCID: PMC2366111. View Article
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