These rat IgM monoclonal antibodies were generated against polysaccharide and recognize land plants arabinogalactan protein (AGP).
Highlights:
Generated to rice arabinogalactan-proteins (AGPs). Recognizes a carbohydrate epitope containing Β-linked glucuronic acid. It can recognize AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-Ο-methyl-Β-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-Ο-methyl-Β-D-GlcA. Arabinogalactans are plant glycans composed primarily of arabinosyl and/or galactosyl residues. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
From the laboratory of Paul Knox, PhD, University of Leeds.
Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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Product Type: | Antibody |
Antigen: | Arabinogalactan-protein (AGP) |
Isotype: | IgM (except JIM8, IgG2c) |
Clonality: | Monoclonal |
Clone Name: | Jim4, 8, 13, 15, 16 and LM2, 14, 30 |
Reactivity: | Land Plants |
Immunogen: | Polysaccharide |
Species Immunized: | Rat |
Epitope: | LM2 and LM14: Bind to Glucuronosyl residue of AGP, LM30: Binds to Arabinosyl residues of AGP, JIM16: Binds to Galactosyl residue of AGP, Others: unknown |
Buffer: | Cell Culture Supernatant, 0.05% Sodium Azide |
Tested Applications: | IF 1:10, ELISA 1:10 (LM2 only) |
Storage: | -20C |
Shipped: | Room Temperature |
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