Ad CMV p53
Ad CMV p53 expresses p53 under control of a CMV promoter.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Ad CMV p53 expresses p53 under control of a CMV promoter.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
Specifications
| Product Type: | Virus |
| Name: | Ad CMV p53 |
| Biosafety Level: | BSL-2 |
| Vector Information: | Recombinant adenovirus |
| Target Gene / Reporter(s): | p53 |
| Promoter: | CMV |
| Virus: | Adenovirus |
| Titer: | 1X10^12 pts/mL, CsCl purified, RCA free and PFU titred |
| Storage: | Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C. |
| Shipped: | Frozen on Dry ice |
Comments
Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.
References
- Anderson RD, Haskell RE, Xia H, Roessler BJ, and Davidson BL. A Simple Method for the Rapid Generation of Recombinant Adenovirus Vectors. Gene Therapy. (2000) 7, 1034-1038.
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