Anti-Exocyst Complex, Exo70 (EXOC7) Subunit [70X13F3] Antibody
This monoclonal antibody recognizes exocyst complex exo70 subunit (EXOC7) and is reactive with mouse and rat.
Exocyst complex (also known as the sec6/8 complex) is a multimeric complex that has been implicated to play a role in exocytosis. This complex is conserved from yeast to mammals and is composed of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84). In the budding yeast Saccharomyces cerevisiae, mutations in these complex subunits promote cytoplasmic accumulation of secretory vesicles and affects polarized growth. In cultured Drosophila and mammlian cells, disruption of exocyst subunit function has been found to decrease neurite outgrowth and Golgi-to-plasma membrane vesicle trafficking. Furthermore, a delay in neuronal induction was observed in mouse embryos with deletion of the sec8 gene, suggesting that the exocyst complex may play a role in cell differentiation and polarized growth.
From the laboratory of Shu-Chan Hsu, PhD, Rutgers University.
This monoclonal antibody recognizes exocyst complex exo70 subunit (EXOC7) and is reactive with mouse and rat.
Exocyst complex (also known as the sec6/8 complex) is a multimeric complex that has been implicated to play a role in exocytosis. This complex is conserved from yeast to mammals and is composed of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84). In the budding yeast Saccharomyces cerevisiae, mutations in these complex subunits promote cytoplasmic accumulation of secretory vesicles and affects polarized growth. In cultured Drosophila and mammlian cells, disruption of exocyst subunit function has been found to decrease neurite outgrowth and Golgi-to-plasma membrane vesicle trafficking. Furthermore, a delay in neuronal induction was observed in mouse embryos with deletion of the sec8 gene, suggesting that the exocyst complex may play a role in cell differentiation and polarized growth.
From the laboratory of Shu-Chan Hsu, PhD, Rutgers University.
| Product Type: | Antibody |
| Name: | Anti-exocyst exo70 subunit (70X13F3) monoclonal antibody |
| Accession ID: | Q9UPT5 |
| Host: | Mouse |
| Isotype: | IgG subtype not characterized |
| Clonality: | Monoclonal |
| Clone Name: | 70X13F3 |
| Specificity: | This antibody recognizes exocyst complex exo70 subunit. Reactive against Mouse and Rat. |
| Immunogen: | Recombinant protein corresponding to full-length rat brain exo70 subunit |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Tested Applications: | Western blotting, ELISA and Immunofluorescence microscopy. |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | Store at -20C |
| Shipped: | Cold packs |
(left) Western blot analysis: 10 ug Rat brain lysate was resolved on a 8% SDS-polyacrylamide gel, transferred to nitrocellulose membrane and probed with 0.01ug/ml antibody.
(right) Immunofluorescence microcopy: Methanol-fixed neuroendocrine PC12 cells were stained with 0.1ug/ml antibody. For best visualization, the cells were incubated with the primary antibody at 4 OC overnight.
- Chernyshova Y, Leshchyns'ka I, Hsu SC, Schachner M, Sytnyk V. (2011) The neural cell adhesion molecule promotes FGFR-dependent phosphorylation and membrane targeting of the exocyst complex to induce exocytosis in growth cones. J. Neurosci 31:3522-3535.
- Liu J, Zuo X, Yue P, Guo W (2007) Phosphatidylinositol 4,5-bisphosphate mediates the targeting of the exocyst to the plasma membrane for exocytosis in mammalian cells. Mol Biol Cell. 18:4483-4492.
- Zuo X, Zhang J, Zhang Y, Hsu SC, Zhou D, Guo W. (2006) Exo70 interacts with the Arp2/3 complex and regulates cell migration. Nat Cell Biol. 2006 8:1383-1388.Ewart MA, Clarke M, Kane S, Chamberlain LH, Gould GW. (2005) Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes. J Biol Chem. 280:3812-3816.
- Vega, I. and Hsu, S.-C. (2001) The Exocyst complex associates with microtubules to mediate vesicle targeting and neurite outgrowth. J. Neurosci. 21: 3839-3848.
- Bernabé-Rubio M, Andrés G, Casares-Arias J, Fernández-Barrera J, Rangel L, Reglero-Real N, Gershlick DC, Fernández JJ, Millán J, Correas I, Miguez DG, Alonso MA. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells. J Cell Biol. 2016 Aug 1;214(3):259-73. doi: 10.1083/jcb.201601020. PubMed PMID: 27458130; PubMed Central PMCID: PMC4970324.View Article
- Miao Y, Wu J, Abraham SN. Ubiquitination of Innate Immune Regulator TRAF3 Orchestrates Expulsion of Intracellular Bacteria by Exocyst Complex. Immunity. 2016 Jul 19;45(1):94-105. View Article
- Bhalla M, Van Ngo H, Gyanwali GC, Ireton K. The Host Scaffolding Protein Filamin A and the Exocyst Complex Control Exocytosis during InlB-Mediated Entry of Listeria monocytogenes. Infect Immun. 2018 Dec 19;87(1). pii: e00689-18. View Article
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