Scot R. Kimball, PhD, Penn State College of Medicine

Scot R. Kimball, PhD
Scot R. Kimball, PhD
mRNA translation is linked to the physiopathological state of cells and tissues. Puromycin, an aminonucleoside antibiotic produced by Streptomyces alboniger, is a structural analog of aminoacyl tRNAs, and can incorporate into the nascent polypeptide chain and prevent elongation. When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro.

 

The Kimball lab has developed a method for using monoclonal antibodies to puromycin to directly monitor translation using standard immunochemical techniques. Pulse-chase analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, allowing for the direct evaluation of translation directly using standard immunochemical methods.

In addition to the puromycin monoclonal antibody, the Kimball Lab has also developed a variety of eukaryotic initiation factor (eIF) monoclonal antibodies.

Products

References

  1. Kelleher AR, Kimball, SR, Dennis MD, Schilder RJ and Jefferson LS. The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb. Am J Physiol Endocrinol Metab. 304(2):E229-236. 2013.
  2. Sukarieh, R., et al. The eIF4E-binding proteins are modifiers of cytoplasmic eIF4E relocalization during the heat shock response. Am J Physiol. 296:C1207-17. 2009.
  3. Rong, L., et al. Control of eIF4E cellular localization by eIF4E-binding proteins, 4E-BPs. RNA. 14: 1318-27. 2008.
  4. Kimball, S.R., et al. A microtiter plate assay for assessing the interaction of eukaryotic initiation factor eIF4E with eIF4G and eIF4E binding protein-1. Anal Biochem. 325: 364-8. 2004.
  5. Kimball, S.R., et al. Mammalian stress granules represent sites of accumulation of stalled translation initiation complexes. Am J Physiol 284: C273 - 84. 2003.
  6. Kimball, S.R., et al. Insulin stimulates protein synthesis in skeletal muscle by enhancing the association of eIF-4E and eIF-4G. Am J Physiol 272: C754 - 9. 1997.
  7. Jamison, J.T., et al. Persistent redistribution of poly-adenylated mRNAs correlates with translation arrest and cell death following global brain ischemia and reperfusion. Neuroscience. 154: 504 - 20. 2008.
  8. Baum, JI et al. Glucagon acts in a dominant manner to repress insulin-induced mammalian target of rapamycin complex 1 signaling in perfused rat liver. Am J Physiol Endocrinol Metab. 297(2): E410 - 5. 2009.