Chad Borges, Ph.D., Arizona State University

The Borges laboratory focuses on the analysis of protein posttranslational modifications as mediators of pathobiology and markers of chronic disease. Mass spectrometric immunoassay is a commonly used tool in the laboratory that allows the targeted immunoaffinity-based extraction of protein(s) from biological milieu and subsequent elution for direct analysis by mass spectrometry. When isolated proteins are eluted from the stationary phase, the resulting solution is often very low in protein concentration (i.e., less than 1 micromolar). If the samples are to be kept in the liquid phase for more than a few minutes two major problems can result: First, low concentration protein solutions are at risk of becoming no-concentration protein solutions due to protein adsorption to vial walls. Second, methionine-containing proteins are susceptible to air-induced sulfoxidation over time, potentially leading to analytical anomalies and difficulties when mass spectrometry is used as a detection modality. The products offered here are designed to overcome these problems and fully stabilize low concentration protein solutions pending analysis by LC-MS.

Part of The Investigator's Annexe program.