The term “click chemistry” was coined to describe a class of efficient and selective reactions that can be used to join molecules together rapidly and in high yield. This class of chemical reactions uses bio-orthogonal or biologically unique moieties to label and join two molecules of interest in a two-step procedure.
By labelling a biological moiety with a fluorescent dye, one can track the progress of the biomolecule within a particular environment and also observe interactions with other entities using an array offluorescent technologies.
Chemoselective Ligation via Copper-free Click Chemistry