Julie P. Deans, PhD, University of Calgary

Julie P. Deans, PhD
Julie P. Deans, PhD

The Deans laboratory has worked principally on B cell antigen receptor signaling mechanisms and the structure and function of CD20 (MS4A1), contributing to the understanding of the topology and extracellular epitopes of CD20, its association with the B cell antigen receptor, role in receptor-activated calcium influx, and its requirement for optimal humoral immunity. More recently, the laboratory has evaluated expression of other members of the MS4A family, focusing mainly on expression of MS4A4A and MS4A8B using monoclonal antibodies made in-house against extracellular epitopes.

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References

  1. Deans, J.P., Robbins, S.R., Polyak, M.J., and Savage, J.A. Rapid Redistribution of CD20 to a Low-density Detergent-insoluble Membrane Compartment. J. Biol. Chem. 273:344-348 (1998)
  2. Polyak, M.J., Tailor, S.H., and Deans, J.P. Identification of a cytoplasmic region of CD20 required for its redistribution to a detergent-insoluble membrane compartment. J. Immunol. 161: 3242-3248 (1998)
  3. Popoff, I.J., and Deans, J.P. Activation and tyrosine phosphorylation of protein kinase C in response to B cell antigen receptor stimulation. Mol. Imm. 36: 1005-1016 (1999)
  4. Petrie R.J., Schnetkamp, P.P.M., Patel, K.D., Awasthi-Kalia, M., and Deans, J.P. Transient translocation of the B cell receptor and SHIP to lipid rafts: Evidence toward a role in calcium regulation. J. Immunol. 165: 1220-1227 (2000)
  5. Petrie, R., and Deans, J.P. Co-localization of the B cell antigen receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts. J. Immunol. 169:2886-2891 (2002)
  6. Polyak, M. J., and Deans, J.P. Alanine-170 and Proline-172 are critical determinants for extracellular CD20 epitopes. Heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. May 1;99(9):3256-62 (2002)
  7. Polyak, M.J., Ayer, L., Szczpek, A. and Deans, J.P. A cholesterol-dependent CD20 epitope detected by the FMC7 antibody Leukemia 17:1384-9 (2003)
  8. Li, H., Ayer, L., Lytton, J., and Deans, J.P. Store-operated cation entry mediated by CD20 in membrane rafts J. Biol. Chem. 278: 42427-42434 (2003)
  9. Li H, Ayer LM, Polyak MJ, Mutch CM, Petrie RJ, Gauthier L, Shariat N, Hendzel MJ, Shaw AR, Patel KD, Deans JP. The CD20 calcium channel is localized to microvilli and constitutively associated with membrane rafts: antibody binding increases the affinity of the association through an epitope-dependent cross-linking-independent mechanism. J Biol Chem. 2004 May 7;279(19):19893-901.
  10. Mutch, C.M., Sanyal, R., Unruh, T.L., Grigoriou, L. and Deans, J. P. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes; evidence for a role in internalization of the B cell receptor. Int Immunol. 19(1):19-30 (2007)
  11. Deans J.P. and Polyak, M.J. FMC7 is an epitope of CD20. Blood Feb 15;111(4):2492 (2008)
  12. Polyak, M.J., Li, H., Shariat, N. and Deans, J. P. CD20 homo-oligomers physically associate with the B cell antigen receptor; dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin binding proteins. J. Biol. Chem. 283: 18545-18552 (2008)
  13. Zuccolo, J., Bau, J., Childs, S.J., Goss, G.G., Sensen, C.W. and Deans, J. P. Phylogenetic Analysis of the MS4A and TMEM176 gene families. PLoS One 5: e9369 1-10 (2010)
  14. Zuccolo, J., Deng, L., Unruh, T.L., Sanyal, R., Bau, J.A., Storek, J., Demetrick, D.J., Luider, J.M., Auer-Grzesiak, I.A., Mansoor, A. and Deans, J.P. Expression of MS4A and TMEM176 genes in human B lymphocytes. Front. Immunol. 4: article 195 (2013)
  15. Morsy, D., Sanyal, R., Zaiss, A.K., Deo, R., Muruve, D.A., and Deans, J.P. Reduced T-dependent humoral Immunity in CD20-deficient mice. J. Immunol. 191:3112-8 (2013)
  16. Sanyal R, Polyak MJ, Zuccolo J, Puri M, Deng L, Roberts L, Zuba A, Storek J, Luider JM, Sundberg EM, Mansoor A, Baigorri E, Chu MP, Belch AR, Pilarski LM, Deans JP. MS4A4A: a novel cell surface marker for M2 macrophages and plasma cells. Immunol Cell Biol. 2017 Apr 11. doi: 10.1038/icb.2017.18.