The long-term research goals of the Wong laboratory are to develop Bacillus subtilis, a gram-positive bacterium, as a microbial factory to produce biotechnologically important proteins via secretion and to design/produce interesting proteins in their natural and engineered forms. While the development and improvement of the secretion and surface display systems are in progress, several interesting proteins including single-chain antibodies, bacterial plasminogen activators (blood-clot dissolving agents) and streptavidin are selected to evaluate the effectiveness of this expression system. Certain novel and desirable properties will be introduced into these molecules through genetic and protein engineering.