FAST Serum Biomarker Chip Kit

The Serum Biomarker Chip kit enables proteomics researchers to profile and pattern the molecular signature of human serum.  The chip addresses the need for a high-throughput technology to enable research in the fields of risk stratification, disease prognosis, drug eligibility, prediction of safety and efficacy, and therapeutic monitoring.

The Serum Biomarker Chip is a single-capture antibody array built on the FAST Slide dual pad platform.  Each slide has two identical arrays of antibodies printed in triplicate. Two-color fluorescent detection permits the researcher to reproducibly pattern the relative abundance of 120 human serum proteins between two samples, such as serum samples from diseased and healthy individuals.

The Serum Biomarker Chip Kit includes two arrayed 2 pad FAST Slides, two dual Incubation Chambers, 5 ml KeraFAST Protein Array Blocking Buffer, and 125 ml KeraFAST Protein Array Wash Buffer.

We offer two protein fluorescent labeling systems:

• ULS Biotin Single Color Labeling System-- for easy, efficient labeling of proteins, especially for single-capture antibody arrays

The KeraFAST Single Color ULS™ Biotin Labeling Kit is designed to label serum or cell lysate proteins in order to detect their binding to complementary antibodies.  The kit is especially intended for use with 2 pad FAST Slides, including the Serum Biomarker Chip and other single-capture antibody chips.  The kit contains Universal Linkage System (ULS) chemistry from KREATECH® Biotechnology to label samples containing approximately 100 to 120 µg of protein in serum or plasma or 60 µg protein in a whole cell lysate.

Features

• Highly efficient and uniform labeling of complex protein samples
• Reproducible labeling and signal detection
• Stable, robust, and fast non enzymatic process
• Broad pH range of labeling
• Compatible with most buffers, salts, detergents, and reducing agents
• Labels multiple amino acids

ULS chemistry tags protein methionine, cysteine and histidine residues with biotin (normally at least two biotins per molecule). The attached biotin is then detected with fluorescent streptavidin.

Two-Color Labeling and Detection System - for dual-label hapten swapping applications

The KeraFAST Two-Color Labeling and Fluorescent Detection Kit is designed to label two protein samples.  The labeled proteins are pooled and probed against arrayed antibodies in a competitive binding assay and detected using indirect fluorescence.
The kit is intended for use with the 2 pad FAST Slides, including the Serum Biomarker Chip. The kit contains the Universal Linkage System (ULS) chemistry to label samples containing approximately 250 µg of protein in serum, plasma or a whole cell lysate. The kit is designed to label two different protein samples, each with a different hapten. Sufficient labeling reagent is provided to perform a hapten swapping experiment.

Features

• Highly efficient and uniform labeling of complex serum samples
• Reproducible labeling and signal detection
• Stable, robust, and fast non enzymatic procedure
• Reduces pH dependency of labeling efficiency
• System solution includes labeling reagents, fluorescent conjugate, and bench-friendly protocol
• Accounts for hapten-specific differences in either Biotin-ULS or Fluorescein-ULS labeling efficiencies
• Averages differences in antibody-antigen binding interactions caused by steric hindrance
• Minimizes chip-to-chip variability – includes an internal control within the assay

The first pad on the slide is probed with a mixture of two different protein samples, each labeled with a different hapten; the second pad is probed with the same two protein samples but with the haptens reversed. The normalized intensity for each element of each pad is calculated as the average of the biotin and fluorescein labeled derived intensities from a two pad experiment. The ratio between the signal intensity at each spot corresponds to the concentration ratio of the proteins found in the two samples. This method is attractive for antibody chips as it takes into account any hapten-specific differences in antigen-antibody interactions.
The use of the ULS labeling system minimizes background by using indirect fluorescence detection, labels multiple amino acids, and requires no additional materials or reagents.

Each kit includes 20 μL Biotin-ULS, 20 μl Fluorescein-ULS, 80 μl 10x Protein Labeling Buffer, 80 μl 10x KREAstop Solution, 150 μl Streptavidin-DY 647 Conjugate, 350 μl Anti-Fluorescein Antibody-DY 547 Conjugate, 8 Micro Bio-Spin Chromatography Columns, and a User's Manual.

Serum Biomarker Chip Datasheet