Anti-Homogalacturonan [CCRC-M38] Antibody (supernatant)
![Anti-Homogalacturonan [CCRC-M38] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1029_2001202001263110280.jpg "Anti-Homogalacturonan [CCRC-M38] Antibody (supernatant)")
This mouse IgG1 monoclonal (CCRC-M38) was generated against seed mucillage/MeBSA complex (Non-covalent) and belongs to the Homogalacturonan (HG) Backbone-1 clade of antibodies and binds to a de-esterified α-1,4 linked Homogalcturonan (HG) epitope (DP>4).
Highlights:
- Reacts with a de-esterified α-1,4 linked Homogalcturonan (HG) epitope (DP>4)
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgG1 monoclonal (CCRC-M38) was generated against seed mucillage/MeBSA complex (Non-covalent) and belongs to the Homogalacturonan (HG) Backbone-1 clade of antibodies and binds to a de-esterified α-1,4 linked Homogalcturonan (HG) epitope (DP>4).
Highlights:
- Reacts with a de-esterified α-1,4 linked Homogalcturonan (HG) epitope (DP>4)
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Accession ID: | CCRC-M38 |
| Antigen: | Homogalacturonan of Arabidopsis thaliana |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | CCRC-M38 |
| Reactivity: | Many plants |
| Immunogen: | Seed mucillage/MeBSA (Non-covalent complex) |
| Species Immunized: | Mouse |
| Epitope: | De-esterified homogalacturonan (DP > 4) |
| Buffer: | Cell culture supernatant, Use undiluted or at 1:10 dilution |
| Tested Applications: | ELISA, Immunolabeling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
CCRC-M38 binds to a fully de-esterified homogalacturonan epitope having a degree of polymerization of 5 or more. CCRC-M38 does not recognize a homogalacturonan trimer.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive tool kit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
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