NIH 3T3 Duo-Parental Cell Line
The NIH 3T3 Duo-Parental Cell Line allows for the direct study of the dynamics of single double-strand breaks (DSBs) and translocations in living mammalian cells in vivo.
NIH3T3 cells which contain integrated into chromosome 7 the ISceI restriction endonuclease site adjacent to an array of the Lac-operator DNA sequence (LacO, 256 copies) and three integrations on chromosomes 1 and 10 (integrations on two chromosome homologs) of the TetO-ISceI-TetO array (TetO, 96 copies). This cell line does not coexpress the fluorescent repressors LacR-GFP and TetR-mCherry.
From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.
Part of The Investigator's Annexe program.
The NIH 3T3 Duo-Parental Cell Line allows for the direct study of the dynamics of single double-strand breaks (DSBs) and translocations in living mammalian cells in vivo.
NIH3T3 cells which contain integrated into chromosome 7 the ISceI restriction endonuclease site adjacent to an array of the Lac-operator DNA sequence (LacO, 256 copies) and three integrations on chromosomes 1 and 10 (integrations on two chromosome homologs) of the TetO-ISceI-TetO array (TetO, 96 copies). This cell line does not coexpress the fluorescent repressors LacR-GFP and TetR-mCherry.
From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.
Part of The Investigator's Annexe program.
This product is for sale to Nonprofit customers only. For profit customers, please Contact Us for more information.
| Product Type: | Cell Line |
| Name: | NIH 3T3 Duo-Parental |
| Cell Type: | NIH3T3 mouse embryonic fibroblasts |
| Accession ID: | CVCL_HC50 |
| Organism: | Mouse |
| Source: | mouse embryo (NIH3T3 cells) |
| Morphology: | Fibroblast |
| Biosafety Level: | BSL1 |
| Subculturing: | Split 1 to 3 when ?90% confluency |
| Growth Conditions: | High-glucose DMEM, 15% FBS, P/S, 2mM Glutamine |
| Cryopreservation: | 15% DMSO in FBS |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
We routinely culture the cells in the absence of the drugs for selection without a major loss of cells containing the arrays.
- Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T. Spatial dynamics of chromosome translocations in living cells. Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
- Roukos V, Burgess RC, Misteli T. Generation of cell-based systems to visualize chromosome damage and translocations in living cells. Nat Protoc. 2014 Oct;9(10):2476-92. doi: 10.1038/nprot.2014.167. Epub 2014 Sep 25.
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