The Tat-GPR peptide was designed to functions as a guanine nucleotide dissociation inhibitor (GDI) that selectively inhibits the Gαi subunit.
Highlights:
Mechanistically, GDIs are well suited to pursue receptor pharmacology of Gαi versus Gβγ signaling and their specific roles in cell signaling and disease. The Tat-GPR peptide stabilizes the GDP-bound conformation of Gαi, prevents the binding of AGS3 to Gαi subunits, blocks receptor coupling to G-protein, and Tat-GPR has shown efficacy in an in vivo model of cocaine addiction and in vitro models of cancer. Tat-GPR peptide at 100nM suppresses α2-AR associated cAMP/Gαi activity in cell culture.
From the laboratory of Yuri K. Peterson, PhD, Medical University of South Carolina.
Product Type: | Protein |
Name: | Tat-GPR |
Accession ID: | P19086, CAS: 419535-56-1 |
Source: | Synthetic chemistry |
Molecular Weight: | 4846.6 Da |
Amino Acid Sequence: | TMGEEDFFDLLAKSQSKRMDDQRVDLAK |
Fusion Tag(s): | GRKKRRQRRRPP |
Format: | Powder |
Purity: | >95% HPLC and MS data |
Solubility: | Water or HEPES buffered water |
Suggested Amount per Experiment: | 300nM (150ng in 100uL) |
Comments: | Working stock=30mM |
Storage: | Long term -20C |
Shipped: | Ambient temperature |
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