Serum-Free DME/F12 Medium
The Serum-Free DME/F12 Medium allows for the growth of a variety of kidney epithelial cell lines as well as primary cultures of kidney tubule epithelial cells, in the absence of fibroblast overgrowth.
Highlights:
- Serum-Free – insulin, transferrin, triiodothyronine (T3), hydrocortisone, prostaglandin E1 (PGE1), and selenium additives allow for robust growth in the absence of FBS
- Shown to work well with Madin-Darby canine kidney (MDCK), M1 mouse collecting duct, human proximal tubule HK1, and opossum kidney OK cell lines, as well as with primary kidney tubule epithelial cells
From the laboratory of Mary L. Taub, PhD, University of Buffalo.
The Serum-Free DME/F12 Medium allows for the growth of a variety of kidney epithelial cell lines as well as primary cultures of kidney tubule epithelial cells, in the absence of fibroblast overgrowth.
Highlights:
- Serum-Free – insulin, transferrin, triiodothyronine (T3), hydrocortisone, prostaglandin E1 (PGE1), and selenium additives allow for robust growth in the absence of FBS
- Shown to work well with Madin-Darby canine kidney (MDCK), M1 mouse collecting duct, human proximal tubule HK1, and opossum kidney OK cell lines, as well as with primary kidney tubule epithelial cells
From the laboratory of Mary L. Taub, PhD, University of Buffalo.
Specifications
| Product Type: | Buffer or Chemical |
| Phenol Red Indicator: | Yes |
| Serum Level: | None |
| Format: | Liquid |
| Components: | Other additives: Insulin, transferrin, T3, hydrocortisone, PGE1, selenium |
| Storage: | Stable for >1yr when unopened and stored at -20C |
| Shipped: | Dry ice |
Documentation
Notes:
Growth factors added to medium at the time of use.
Provider
From the laboratory of Mary L. Taub, PhD, University of Buffalo.
References
- Taub M, Chuman L, Saier MH Jr, Sato G. Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3338-42.
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