Anti-Beta-Spectrin, C-Terminal [SP316] Antibody
![Anti-Beta-Spectrin, C-Terminal [SP316] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1209_2001202001263812080.jpg "Anti-Beta-Spectrin, C-Terminal [SP316] Antibody")
This mouse IgG monoclonal antibody were generated against recombinant peptide (residues 1902-2037 fused with GST) and is specific for human red blood cell beta-spectrin isoform (alternatively spliced C-terminal), residues 2085-2137.
Highlights:
- Reacts with human red blood cell beta-spectrin isoform (alternatively spliced C-terminal)
- Capable of recognizing the C-terminal region of β-spectrin regardless of its phosphorylation state
- Used it to purify the phosphorylated region after 2-nitro-5-thiocyanobenzoic acid cleavage of spectrin
- Recommended for Western Blot applications
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Beta-Spectrin functions as a structural element and stabilizes erythrocyte membranes. Mutations in beta-spectrin are described as a possible cause of neonatal hemolytic anemia, spherocytosis type 2, and hereditary elliptocytosis.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
This mouse IgG monoclonal antibody were generated against recombinant peptide (residues 1902-2037 fused with GST) and is specific for human red blood cell beta-spectrin isoform (alternatively spliced C-terminal), residues 2085-2137.
Highlights:
- Reacts with human red blood cell beta-spectrin isoform (alternatively spliced C-terminal)
- Capable of recognizing the C-terminal region of β-spectrin regardless of its phosphorylation state
- Used it to purify the phosphorylated region after 2-nitro-5-thiocyanobenzoic acid cleavage of spectrin
- Recommended for Western Blot applications
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane in eukaryotic cells along with actin. Spectrin plays a role in cell shape control, arrangement of transmembrane proteins, and organelle positioning. Beta-Spectrin functions as a structural element and stabilizes erythrocyte membranes. Mutations in beta-spectrin are described as a possible cause of neonatal hemolytic anemia, spherocytosis type 2, and hereditary elliptocytosis.
From the laboratories of David W. Speicher, PhD, The Wistar Institute.
| Product Type: | Antibody |
| Antigen: | Human red blood cell beta-spectrin isoform (alternatively spliced C-terminal) |
| Accession ID: | P11277 |
| Molecular Weight: | 246 kDa |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: | SP316 |
| Reactivity: | Human |
| Immunogen: | Recombinant peptide (residues 1902-2037 fused with GST) |
| Species Immunized: | Mouse |
| Epitope: | Residues 2085-2137 |
| Purification Method: | Affinity purified |
| Tested Applications: | Western Blot (1:250,000; 20sec exposure) |
| Storage: | -20C |
| Shipped: | Cold packs |
Western Blot
Lane 1, Human Melanoma (1205 LU); Lane 2, Human Ovarian Cancer (RMG1); Lane 3, Human Ovarian Cancer (OV90); Lane 4, Human Red Cell Membrane. Gel loads: Lanes 1-3, 25mg; Lane 4, 5mg. Antibody dilution: 1:250,000, 20sec exposure.
- Tang HY, Speicher DW. In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner. Biochemistry. 2004 Apr 13;43(14):4251-62.
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