Anti-Tomato Xyloglucan [CCRC-M96] Antibody (supernatant)

Anti-Tomato Xyloglucan [CCRC-M96] Antibody (supernatant)

This mouse IgG3 monoclonal antibody was generated against xyloglucan and recognizes tomato (Lycopersicon esculentum) xyloglucan.

Highlights:

  • Reacts with tomato, tamarind and linseed xyloglucan
  • Binds to non-fucosylated xyloglucans
  • Suitable for ELISA applications

Xyloglucan is a hemiceullose or polysaccharide that is found in the primary cell wall of all vascular plants. Xyloglucan binds to the surface of cellulose microfibrils and may link them together.

From the laboratory of Michael G. Hahn, PhD, University of Georgia.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA133
Anti-Tomato Xyloglucan [CCRC-M96] Antibody (supernatant)
5mL (supernatant) 4-6 weeks
Regular Price:$310.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Tomato (Lycopersicon esculentum)xyloglucan
Isotype: IgG3
Clonality: Monoclonal
Clone Name: 14D2.H3.A5.A5
Reactivity: Tomato, tamarind, linseed
Immunogen: Xyloglucan
Species Immunized: Mouse
Epitope: XLLG
Buffer: Cell culture supernatant
Tested Applications: ELISA
Storage: <1 month at 4C, >1 month at -80C
Shipped: Cold Packs

Documentation

PDF CCRC-M96 Cross Reactivity Map

Notes:

These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).

Provider
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
References
  1. Robin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn and A. Lacey Samuels. 2008. Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage. The Plant Cell June 2008 vol. 20 no. 6 1623-1638 .
  2. DeMartini, JD, Pattathil, S, Avci, U, Szekalski, K, Mazumder, K, Hahn, M.G., Wyman, CE: Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production. Energy Environ. Sci., 2011, 4, 4332-4339.
  3. Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
  4. A.P. de Souza, D.C.C. Leite, S. Pattahil, M.G. Hahn, M.S. Buckeridge. 2013. Composition and structure of sugarcane cell wall polysaccharides: Implications for second generation bioethanol production. Bioenergy Research 6: 564-579.
  5. J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.
  6. G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.
  7. G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.
  8. Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
  9. Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
  10. Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.

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